| Name |
IgG3 ELISA Kit (Human) (GWB-KBBTL8) |
| Kit Range |
0.156-10 ng/mL |
| Sensitivity |
< 0.055 ng/mL |
| Sample Type |
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernatant |
| Kit Detection |
Absorbance, OD 450 nm |
| Gene Symbol |
IgG3 |
| Gene id |
3502 |
| Alias Symbols |
DKFZp686H11213, FLJ39988, FLJ40036, FLJ40253, FLJ40587, FLJ40789, FLJ40834, HDC, Heavy chain disease protein, IgG3, Ig gamma-3 chain C region, MGC45809 |
| Swissprot ID |
P01860 |
| Protein Name |
IgG3 |
| Kit Principle |
Genway Biotech IgG3 ELISA Kit (Human) (GWB-KBBTL8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IgG3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IgG3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IgG3 captured in the well. |
| Kit Component |
| Component | Amount |
| Anti-IgG3 Microplate | 96 Wells (12 x 8 Well Strips) |
| IgG3 Lyophlized Standard | 2 x 10 ng |
| Standard Diluent | 1 x 20 mL |
| 100X IgG3 Biotinylated Detector Antibody | 1 x 120 µL |
| 100X Avidin-HRP Detection Conjugate | 1 x 120 µL |
| Detector Antibody Diluent | 1 x 12 mL |
| Detection Conjugate Diluent | 1 x 12 mL |
| 10X Sample Diluent | 1 x 30 mL |
| 30X Wash Buffer | 1 x 20 mL |
| TMB Substrate | 1 x 9 mL |
| Stop Solution | 1 x 6 mL | |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3.5 Hours |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBTL8 |
| Species Reactivity |
Human |
