IgG3 ELISA Kit (Human) (GWB-KBBTL8)

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96 Wells
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Additional Information

Name IgG3 ELISA Kit (Human) (GWB-KBBTL8)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.055 ng/mL
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernatant
Kit Detection Absorbance, OD 450 nm
Gene Symbol IgG3
Gene id 3502
Alias Symbols DKFZp686H11213, FLJ39988, FLJ40036, FLJ40253, FLJ40587, FLJ40789, FLJ40834, HDC, Heavy chain disease protein, IgG3, Ig gamma-3 chain C region, MGC45809
Swissprot ID P01860
Protein Name IgG3
Kit Principle Genway Biotech IgG3 ELISA Kit (Human) (GWB-KBBTL8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IgG3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IgG3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IgG3 captured in the well.
Kit Component
Anti-IgG3 Microplate96 Wells (12 x 8 Well Strips)
IgG3 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X IgG3 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBTL8
Species Reactivity Human