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96 Wells
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Additional Information

Name IREM1 ELISA Kit (Human) (GWB-KBBIS4)
Description of Target This gene encodes a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. The encoded protein is an inhibitory receptor
Kit Range 0.156-10 ng/mL
Sensitivity < 0.062 ng/mL
Sample Type Serum, plasma, tissue homogenates, cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol IREM1
Gene id 146722
Alias Symbols CD300 antigen-like family member F, CD300f, CD300F, CLM1, CLM-1, CMRF35-like molecule 1, IgSF13, IGSF13, Immune receptor expressed on myeloid cells 1, Immunoglobulin superfamily member 13, IREM1, IREM-1, NK inhibitory receptor, NKIR, UNQ3105/PRO10111
Swissprot ID Q8TDQ1
Protein Name CMRF35-like molecule 1
Kit Principle Genway Biotech IREM1 ELISA Kit (Human) (GWB-KBBIS4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IREM1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IREM1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IREM1 captured in the well.
Kit Component
Anti-IREM1 Microplate96 Wells (12 x 8 Well Strips)
IREM1 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X IREM1 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001276011.1
Nucleotide Accession Num NM_001289082.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBIS4
Species Reactivity Human