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Additional Information

Name JAG2 ELISA Kit (Human) (GWB-KBBMS3)
Description of Target Putative Notch ligand involved in the mediation of Notch signaling. Involved in limb development (
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.118 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol JAG2
Gene id 3714
Alias Symbols hJ2, HJ2, Jagged2, Protein jagged-2, SER2
Swissprot ID Q9Y219
Protein Name protein jagged-2
Kit Principle Genway Biotech JAG2 ELISA Kit (Human) (GWB-KBBMS3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for JAG2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for JAG2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample JAG2 captured in the well.
Kit Component
Anti-CCT3 Microplate96 Wells (12 x 8 Well Strips)
CCT3 Lyophilized Standard2 x 20 ng
100X Biotinylated CCT3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_002217
Nucleotide Accession Num NM_002226
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)84-94%84-98%91-99%97-104%
EDTA Plasma (n=5)78-97%84-91%87-95%80-97%
Heparin Plasma (n=5)84-92%92-101%86-97%91-98%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)91-10194
EDTA Plasma (n=5)79-10497
Heparin Plasma (n=5)98-105101
Datasheets/Manuals Printable datasheet for GWB-KBBMS3
Species Reactivity Human