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96 Wells
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Additional Information

Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.115 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol KAZALD1
Gene id 81621
Alias Symbols BONO1, FKSG28, FKSG40, FLJ24094, IGFBP-rP10, Kazal-type serine protease inhibitor domain-containing protein 1, UNQ2945/PRO21184
Swissprot ID Q96I82
Protein Name kazal-type serine protease inhibitor domain-containing protein 1
Description of Target Involved in the proliferation of osteoblasts during bone formation and bone regeneration. Promotes matrix assembly (
Kit Principle Genway Biotech KAZALD1 ELISA Kit (Human) (GWB-KBBPA6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KAZALD1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KAZALD1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KAZALD1 captured in the well.
Kit Component
Anti-Ndrg2 Microplate96 Wells (12 x 8 Well Strips)
Ndrg2 Lyophilized Standard2 x 20 ng
100X Biotinylated Ndrg2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001306232
Nucleotide Accession Num NM_001319303
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBPA6
Species Reactivity Human