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KPNB1 ELISA Kit (Human) (GWB-KBBXA1)

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GWB-KBBXA1

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Additional Information

Name KPNB1 ELISA Kit (Human) (GWB-KBBXA1)
Description of Target Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autono
Kit Range 0.781 - 50 ng/mL
Sensitivity < 0.28 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol KPNB1
Gene id 3837
Alias Symbols IMB1, Impnb, Importin-90, Importin subunit beta-1, IPO1, IPOB, Karyopherin subunit beta-1, MGC2155, MGC2156, MGC2157, NTF97, Nuclear factor p97, Pore targeting complex 97 kDa subunit, PTAC97
Swissprot ID Q14974
Protein Name importin subunit beta-1
Kit Principle Genway Biotech KPNB1 ELISA Kit (Human) (GWB-KBBXA1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KPNB1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KPNB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KPNB1 captured in the well.
Kit Component
ComponentAmount
Anti-TRPV3 Microplate96 Wells (12 x 8 Well Strips)
TRPV3 Lyophilized Standard2 x 50 ng
100X Biotinylated TRPV3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001263382
Nucleotide Accession Num NM_001276453
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)79-93%87-101%88-102%89-103%
EDTA Plasma (n=5)97-105%78-96%80-89%93-101%
Heparin Plasma (n=5)97-105%80-101%78-104%79-90%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)86-9592
EDTA Plasma (n=5)80-9592
Heparin Plasma (n=5)84-9288
Datasheets/Manuals Printable datasheet for GWB-KBBXA1
Species Reactivity Human