KRT33A ELISA Kit (Human) (GWB-KBBLW1)

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GWB-KBBLW1

96 Wells
$729.00
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Additional Information

Name KRT33A ELISA Kit (Human) (GWB-KBBLW1)
Kit Range 78.12 - 5,000 pg/mL
Sensitivity < 29 pg/mL
Sample Type serum, plasma and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol KRT33A
Gene id 3883
Alias Symbols HA3I, Ha-3I, Hair keratin, type I Ha3-I, hHa3-I, HHA3-I, HKA3A, K33A, Keratin, type I cuticular Ha3-I, Keratin-33A, Krt1-3, KRTHA3A
Swissprot ID O76009
Protein Name keratin, type I cuticular Ha3-I
Description of Target This gene encodes a member of the keratin gene family. This gene is one of multiple type I hair keratin genes that are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. As a type I hair keratin, the encoded protei
Kit Principle Genway Biotech KRT33A ELISA Kit (Human) (GWB-KBBLW1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KRT33A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KRT33A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KRT33A captured in the well.
Kit Component
ComponentAmount
Anti-KRT7 Microplate96 Wells (12 x 8 Well Strips)
KRT7 Lyophilized Standard2 x 5 ng
100X Biotinylated KRT7 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_004129
Nucleotide Accession Num NM_004138
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)95-103%93-103%90-97%94-101%
EDTA Plasma (n=5)82-90%83-91%78-96%95-105%
Heparin Plasma (n=5)79-89%87-104%88-102%85-97%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)78-9995
EDTA Plasma (n=5)89-10399
Heparin Plasma (n=5)79-9187
Datasheets/Manuals Printable datasheet for GWB-KBBLW1
Species Reactivity Human