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96 Wells
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Additional Information

Kit Range 0.156-10 ng/mL
Sensitivity < 0.055 ng/mL
Sample Type Serum, plasma, saliva, tears
Kit Detection Absorbance, OD 450 nm
Gene Symbol LACRT
Gene id 90070
Alias Symbols Extracellular glycoprotein lacritin, LACRITIN
Swissprot ID Q9GZZ8
Protein Name Extracellular glycoprotein lacritin
Description of Target The protein encoded by this gene is highly expressed in the lacrimal glands and localized primarily to secretory granules and secretory fluid. It augments lacrimal acinar cell secretion, promotes ductal cell proliferation, and stimulates signaling through
Kit Principle Genway Biotech LACRT ELISA Kit (Human) (GWB-KBBQX3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LACRT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LACRT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LACRT captured in the well.
Kit Component
Anti-LACRT Microplate96 Wells (12 x 8 Well Strips)
LACRT Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X LACRT Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_150593.1
Nucleotide Accession Num NM_033277.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBQX3
Species Reactivity Human