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96 Wells
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Additional Information

Name LAMA2 ELISA Kit (Human) (GWB-KBBEW9)
Kit Range 3.12 - 200 ng/mL
Sensitivity < 1.34 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol LAMA2
Gene id 3908
Alias Symbols Laminin-12 subunit alpha, Laminin-2 subunit alpha, Laminin-4 subunit alpha, Laminin M chain, Laminin subunit alpha-2, LAMM, Merosin heavy chain
Swissprot ID P24043
Protein Name Laminin subunit alpha-2
Description of Target Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Kit Principle Genway Biotech LAMA2 ELISA Kit (Human) (GWB-KBBEW9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LAMA2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LAMA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LAMA2 captured in the well.
Kit Component
Anti-LAMA2 Microplate96 Wells (12 x 8 Well Strips)
LAMA2 Lyophilized Standard2 x 200 ng
100X Biotinylated LAMA2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_000417
Nucleotide Accession Num NM_000426
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)78-102%82-95%97-104%84-91%
EDTA Plasma (n=5)90-102%90-98%97-105%83-97%
Heparin Plasma (n=5)82-97%83-97%79-93%80-103%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)83-9787
EDTA Plasma (n=5)80-9994
Heparin Plasma (n=5)80-10299
Datasheets/Manuals Printable datasheet for GWB-KBBEW9
Species Reactivity Human