LAMA4 ELISA Kit (Human) (GWB-KBBQG2)

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GWB-KBBQG2

96 Wells
$659.00
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Additional Information

Name LAMA4 ELISA Kit (Human) (GWB-KBBQG2)
Kit Range 0.625 - 40 ng/mL
Sensitivity < 0.248 ng/mL
Sample Type serum, plasma and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol LAMA4
Gene id 3910
Alias Symbols DKFZp686D23145, LAMA3, LAMA4*-1, Laminin-14 subunit alpha, Laminin-8 subunit alpha, Laminin-9 subunit alpha, Laminin subunit alpha-4
Swissprot ID Q16363
Protein Name Laminin subunit alpha-4
Description of Target Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Kit Principle Genway Biotech LAMA4 ELISA Kit (Human) (GWB-KBBQG2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LAMA4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LAMA4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LAMA4 captured in the well.
Kit Component
ComponentAmount
Anti-LAMA4 Microplate96 Wells (12 x 8 Well Strips)
LAMA4 Lyophilized Standard2 x 40 ng
100X Biotinylated LAMA4 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001098676
Nucleotide Accession Num NM_001105206
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)80-91%92-101%82-99%96-103%
EDTA Plasma (n=5)80-102%99-105%80-97%86-104%
Heparin Plasma (n=5)87-94%94-105%79-94%97-104%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)78-9691
EDTA Plasma (n=5)80-10298
Heparin Plasma (n=5)89-9794
Datasheets/Manuals Printable datasheet for GWB-KBBQG2
Species Reactivity Human