LASS2 ELISA Kit (Human) (GWB-KBBPM6) (Aviva Cat. No. OKDD00370)

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GWB-KBBPM6

Aviva Cat. No.: OKDD00370

Additional Information

Name LASS2 ELISA Kit (Human) (GWB-KBBPM6)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.061 ng/mL
Sample Type Serum, plasma, tissue homogenates, cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol LASS2
Gene id 29956
Alias Symbols CerS2, FLJ10243, L3, LAG1 longevity assurance homolog 2, MGC987, SP260, TMSG1, Tumor metastasis-suppressor gene 1 protein
Swissprot ID Q96G23
Protein Name Ceramide synthase 2
Description of Target This gene encodes a protein that has sequence similarity to yeast longevity assurance gene 1. Mutation or overexpression of the related gene in yeast has been shown to alter yeast lifespan. The human protein may play a role in the regulation of cell growt
Kit Principle Genway Biotech LASS2 ELISA Kit (Human) (GWB-KBBPM6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LASS2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LASS2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LASS2 captured in the well.
Kit Component
ComponentAmount
Anti-LASS2 Microplate96 Wells (12 x 8 Well Strips)
LASS2 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X LASS2 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_071358.1
Nucleotide Accession Num NM_022075.4
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBPM6
Species Reactivity Human