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LAT1 ELISA Kit (Human) (GWB-KBBEM6)

GWB-KBBEM6

96 Wells
$605.00
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Additional Information

Name LAT1 ELISA Kit (Human) (GWB-KBBEM6)
Gene Symbol LAT1
Gene id 8140
Alias Symbols 4F2LC, 4F2 LC, 4F2 light chain, CD98, CD98LC, CD98 light chain, D16S469E, E16, hLAT1, Integral membrane protein E16, Large neutral amino acids transporter small subunit 1, LAT1, L-type amino acid transporter 1, MPE16, Solute carrier family 7 member 5, y+
Protein Name Large neutral amino acids transporter small subunit 1
Swissprot ID Q01650
Protein Accession Num NP_003477.4
Nucleotide Accession Num NM_003486.5
Species Reactivity Human
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Sensitivity < 0.056 ng/mL
Kit Range 0.156-10 ng/mL
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates
Datasheets / Downloads GWB-KBBEM6 Datasheet
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Principle Genway Biotech LAT1 ELISA Kit (Human) (GWB-KBBEM6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LAT1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LAT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LAT1 captured in the well.
Kit Component
ComponentAmount
Anti-LAT1 Microplate96 Wells (12 x 8 Well Strips)
LAT1 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X LAT1 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Absorbance, OD 450 nm
Kit Duration ~ 3.5 Hours