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LDB1 ELISA Kit (Human) (GWB-KBBVY6)

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GWB-KBBVY6

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Additional Information

Name LDB1 ELISA Kit (Human) (GWB-KBBVY6)
Description of Target Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. Plays a role in the development of interneurons an
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.061 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol LDB1
Gene id 8861
Alias Symbols Carboxyl-terminal LIM domain-binding protein 2, CLIM2, CLIM-2, hLdb1, LDB-1, LIM domain-binding factor CLIM2, LIM domain-binding protein 1, NLI, Nuclear LIM interactor
Swissprot ID Q86U70
Protein Name LIM domain-binding protein 1
Kit Principle Genway Biotech LDB1 ELISA Kit (Human) (GWB-KBBVY6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LDB1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LDB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LDB1 captured in the well.
Kit Component
ComponentAmount
Anti-BMPER Microplate96 Wells (12 x 8 Well Strips)
BMPER Lyophilized Standard2 x 10 ng
100X Biotinylated BMPER Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001106878
Nucleotide Accession Num NM_001113407
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBVY6
Species Reactivity Human