| Name |
LECT1 ELISA Kit (Human) (GWB-KBBEL2) |
| Kit Range |
0.156-10 ng/mL |
| Sensitivity |
< 0.051 ng/mL |
| Sample Type |
Serum, Plasma, Tissue homogenates |
| Kit Detection |
Absorbance, OD 450 nm |
| Gene Symbol |
LECT1 |
| Gene id |
11061 |
| Alias Symbols |
BRICD3, CHM1, CHMI, ChM-I, CHM-I, chondromodulin, Chondromodulin-1, Chondromodulin-I, Leukocyte cell-derived chemotaxin 1, MYETS1 |
| Swissprot ID |
O75829 |
| Protein Name |
leukocyte cell-derived chemotaxin 1 |
| Description of Target |
This gene encodes a glycosylated transmembrane protein that is cleaved to form a mature, secreted protein. The N-terminus of the precursor protein shares characteristics with other surfactant proteins and is sometimes called chondrosurfactant protein alth |
| Kit Principle |
Genway Biotech LECT1 ELISA Kit (Human) (GWB-KBBEL2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LECT1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LECT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LECT1 captured in the well. |
| Kit Component |
| Component | Amount |
| Anti-LECT1 Microplate | 96 Wells (12 x 8 Well Strips) |
| LECT1 Lyophlized Standard | 2 x 10 ng |
| Standard Diluent | 1 x 20 mL |
| 100X LECT1 Biotinylated Detector Antibody | 1 x 120 µL |
| 100X Avidin-HRP Detection Conjugate | 1 x 120 µL |
| Detector Antibody Diluent | 1 x 12 mL |
| Detection Conjugate Diluent | 1 x 12 mL |
| 10X Sample Diluent | 1 x 30 mL |
| 30X Wash Buffer | 1 x 20 mL |
| TMB Substrate | 1 x 9 mL |
| Stop Solution | 1 x 6 mL | |
| Protein Accession Num |
NP_001011705.1 |
| Nucleotide Accession Num |
NM_001011705.1 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3.5 Hours |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBEL2 |
| Species Reactivity |
Human |
