LECT1 ELISA Kit (Human) (GWB-KBBEL2) (Aviva Cat. No. OKDD00374)

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GWB-KBBEL2

Aviva Cat. No.: OKDD00374

Additional Information

Name LECT1 ELISA Kit (Human) (GWB-KBBEL2)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.051 ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol LECT1
Gene id 11061
Alias Symbols BRICD3, CHM1, CHMI, ChM-I, CHM-I, chondromodulin, Chondromodulin-1, Chondromodulin-I, Leukocyte cell-derived chemotaxin 1, MYETS1
Swissprot ID O75829
Protein Name leukocyte cell-derived chemotaxin 1
Description of Target This gene encodes a glycosylated transmembrane protein that is cleaved to form a mature, secreted protein. The N-terminus of the precursor protein shares characteristics with other surfactant proteins and is sometimes called chondrosurfactant protein alth
Kit Principle Genway Biotech LECT1 ELISA Kit (Human) (GWB-KBBEL2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LECT1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LECT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LECT1 captured in the well.
Kit Component
ComponentAmount
Anti-LECT1 Microplate96 Wells (12 x 8 Well Strips)
LECT1 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X LECT1 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001011705.1
Nucleotide Accession Num NM_001011705.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBEL2
Species Reactivity Human