LECT1 ELISA Kit (Human) (GWB-KBBJX2) (Aviva Cat. No. OKCD00761)

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Please see item number OKCD00761 to view product details and for ordering information.


GWB-KBBJX2

Aviva Cat. No.: OKCD00761

Additional Information

Name LECT1 ELISA Kit (Human) (GWB-KBBJX2)
Kit Range 31.25 - 2,000 pg/mL
Sensitivity < 12.8 pg/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol LECT1
Gene id 11061
Alias Symbols BRICD3, CHM1, CHMI, ChM-I, CHM-I, chondromodulin, Chondromodulin-1, Chondromodulin-I, Leukocyte cell-derived chemotaxin 1, MYETS1
Swissprot ID O75829
Protein Name leukocyte cell-derived chemotaxin 1
Description of Target Bifunctional growth regulator that stimulates the growth of cultured chondrocytes in the presence of basic fibroblast growth factor (FGF) but inhibits the growth of cultured vascular endothelial cells. May contribute to the rapid growth of cartilage and v
Kit Principle Genway Biotech LECT1 ELISA Kit (Human) (GWB-KBBJX2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LECT1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LECT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LECT1 captured in the well.
Kit Component
ComponentAmount
Anti-STC1 Microplate96 Wells (12 x 8 Well Strips)
STC1 Lyophilized Standard2 x 2 ng
100X Biotinylated STC1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001011705
Nucleotide Accession Num NM_001011705
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)82-96%95-103%85-104%81-91%
EDTA Plasma (n=5)95-105%78-89%79-99%95-102%
Heparin Plasma (n=5)79-101%80-93%78-93%81-101%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)81-8986
EDTA Plasma (n=5)89-9693
Heparin Plasma (n=5)85-9289
Datasheets/Manuals Printable datasheet for GWB-KBBJX2
Species Reactivity Human