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LEPREL1 ELISA Kit (Human) (GWB-KBBXV2)

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GWB-KBBXV2

96 Wells
$709.00
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Additional Information

Name LEPREL1 ELISA Kit (Human) (GWB-KBBXV2)
Description of Target This gene encodes a member of the prolyl 3-hydroxylase subfamily of 2-oxo-glutarate-dependent dioxygenases. These enzymes play a critical role in collagen chain assembly, stability and cross-linking by catalyzing post-translational 3-hydroxylation of prol
Kit Range 0.156-10 ng/mL
Sensitivity < 0.063 ng/mL
Sample Type Serum, plasma, tissue homogenates, cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol LEPREL1
Gene id 55214
Alias Symbols FLJ10718, Leprecan-like protein 1, MLAT4, Myxoid liposarcoma-associated protein 4, P3H2, Prolyl 3-hydroxylase 2
Swissprot ID Q8IVL5
Protein Name Prolyl 3-hydroxylase 2
Kit Principle Genway Biotech LEPREL1 ELISA Kit (Human) (GWB-KBBXV2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LEPREL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LEPREL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LEPREL1 captured in the well.
Kit Component
ComponentAmount
Anti-LEPREL1 Microplate96 Wells (12 x 8 Well Strips)
LEPREL1 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X LEPREL1 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001127890.1
Nucleotide Accession Num NM_001134418.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBXV2
Species Reactivity Human