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LY9 ELISA Kit (Human) (GWB-KBBKO9)

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Additional Information

Name LY9 ELISA Kit (Human) (GWB-KBBKO9)
Description of Target Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are i
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.128 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol LY9
Gene id 4063
Alias Symbols CD229, CDABP0070, Cell surface molecule Ly-9, hly9, Lymphocyte antigen 9, mLY9, SLAMF3, T-lymphocyte surface antigen Ly-9
Swissprot ID Q9HBG7
Protein Name T-lymphocyte surface antigen Ly-9
Kit Principle Genway Biotech LY9 ELISA Kit (Human) (GWB-KBBKO9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LY9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LY9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LY9 captured in the well.
Kit Component
Anti-Stat1 Microplate96 Wells (12 x 8 Well Strips)
Stat1 Lyophilized Standard2 x 20 ng
100X Biotinylated Stat1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001028839
Nucleotide Accession Num NM_001033667
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBKO9
Species Reactivity Human