pOET-2 transfer plasmid (10ug)

GWB-65CD85

44-613-375028

0.01 mg
$254.70
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Description

pOET1 and 2 are baculovirus transfer plasmids designed for high level expression of foreign genes under the powerful AcMNPV polyhedrin (polh) promoter. They have a Col E1 origin of replication and an ampicillin resistance gene for selection in E. coli. The vectors are smaller than other available transfer vectors (4541 bp) which greatly facilitates the cloning steps. The polh coding sequences have been replaced by a multiple cloning site containing 14 unique restriction sites for insertion of the foreign gene in the correct orientation. pOET2 has the same MCS as pOET1, but in the reverse orientation. The PacI site at the end of the MCS provides translational stop codons in all three reading frames for expression of truncated proteins. The AcMNPV sequences flanking the MCS facilitate recombination with the virus DNA to insert the expression cassette into the polh locus. As well as our own flashBAC platform these vectors can also be used with any other baculovirus system that uses homologous recombination to insert the foreign gene into the virus genome.

Additional Information

Name pOET-2 transfer plasmid (10ug)
Related Product Names pOET-2 transfer plasmid (10ug)
Datasheets / Downloads GWB-65CD85 Datasheet
Intended Use Research Use Only