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Molecular Biology Products

We have 36 Molecular Biology Products

FlashBAC™ is a major new platform technology enabling the fast and simultaneous production of multiple recombinant viruses, lending itself to use for high-throughput systems. A selection of transfer plasmids (pOET) are available that facilitate simple, easy cloning into the flashBAC system and can offer further improvements in protein expression.

Items 11 to 20 of 36 total

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  1. $3,014.10
    GenWay ID: GWB-68AE3C
    Legacy ID: 44-613-375018
    Item Type: Molecular Biology Products, Oxford Expression Technologies.

  2. $768.60
    GenWay ID: GWB-6AB80F
    Legacy ID: 44-613-375007
    Item Type: Molecular Biology Products, Oxford Expression Technologies.

  3. $4,698.00
    GenWay ID: GWB-6C34E3
    Legacy ID: 44-613-375023
    Item Type: Molecular Biology Products, Oxford Expression Technologies.

  4. $613.80
    GenWay ID: GWB-7676BB
    Legacy ID: 44-613-375001
    Item Type: Molecular Biology Products, Oxford Expression Technologies.
    Application(s): To be provided by the user:
    Transfection Reagent - reagents tested and found to be successful: baculoFECTIN II© (OET), Lipofectin© (Invitrogen), FuGENE 6 (Promega), GeneJuice© (Novagen)
    Transfection media (e.g. Grace's Insect Basal Medium or TC100)
    35mm tissue culture treated dishes seeded with Sf21 (1.4 x 106 cells/2mls), or Sf9 (1 x 106 cells/2mls) per dish
    Prepare 2 hours before transfection
    Sterile baculovirus transfer vector DNA containing gene of interest (500ng per co-transfection)

    Procedure: (as recommended with use of baculFECTIN II. Other reagents may differ.)
    Prepare DNA transfection complexes
    1. Warm baculoFECTIN II to RT and vortex gently.
    2. Place 100ul transfection medium in a sterile tube.
    3. Add 5ul (100ng) co-transfection factor. Tap tube to mix.
    4. Add 5ul (500ng) plasmid transfer vector. Tap to mix.
    5. Add 1.2ul of baculFECTIN II. Tap to mix.
    6. Incubate at RT for 15-20 minutes.
    Add DNA complexes to cells.
    1. Remove 1mL medium from each dish required.
    2. Add baculoFECTIN II:DNA complex mixture drop-wise to different areas of the dish.
    3. Incubate 16-24 hours.
    4. Add 1mL fresh medium to each culture dish.
    5. Incubate for 4 days at 28°C (5 days from start of co-transfection).
    Harvest P0 virus stock
    1. Aspirate medium from dishes and remove cells at 3000 rpm for 10 minutes.
    2. Amplify virus stock.

  5. $717.30
    GenWay ID: GWB-97BE6D
    Legacy ID: 44-613-375003
    Item Type: Molecular Biology Products, Oxford Expression Technologies.

  6. $883.80
    GenWay ID: GWB-B05C47
    Legacy ID: 44-613-375011
    Item Type: Molecular Biology Products, Oxford Expression Technologies.

  7. $1,247.40
    GenWay ID: GWB-B95CAC
    Legacy ID: 44-613-375031
    Item Type: Molecular Biology Products, Oxford Expression Technologies.

  8. $2,286.90
    GenWay ID: GWB-DC7364
    Legacy ID: 44-613-375008
    Item Type: Molecular Biology Products, Oxford Expression Technologies.

  9. $2,541.60
    GenWay ID: GWB-E70A54
    Legacy ID: 44-613-375014
    Item Type: Molecular Biology Products, Oxford Expression Technologies.

  10. $1,600.20
    GenWay ID: GWB-EB1FF8
    Legacy ID: 44-613-375002
    Item Type: Molecular Biology Products, Oxford Expression Technologies.

Items 11 to 20 of 36 total

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