Albumin ELISA Kit (Mouse) (GWB-282C17)

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GWB-282C17

40-374-130046

96 Wells
$329.00
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Description

Short Description: In this assay the albumin present in serum sample reacts with the anti-Alb. antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound serum proteins by washing, anti-Alb. antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound serum Alb. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Albumin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Albumin in the test sample. The quantity of Albumin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for serum dilution.

Additional Information

Name Albumin ELISA Kit (Mouse) (GWB-282C17)
Related Product Names Mouse Albumin ELISA Kit; N/A Mouse AlbuminAlb, Alb1
Kit Range 7.8 ng/ml - 500 ng/ml
Sensitivity 1.704 ng/ml
Sample Type Serum, plasma and other biological fluids
Gene Symbol ALB
Gene Fullname albumin
Gene id 11657
Alias Symbols Albumin, ALB
Swissprot ID P07724
Protein Name Serum albumin
Kit Principle Genway Biotech Albumin ELISA Kit (Mouse) (GWB-282C17) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Serum albumin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Serum albumin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serum albumin captured in the well.
Kit Component
ComponentAmount
Anti-Mouse Albumin Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Mouse Albumin Calibrator1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Homology Mouse
NCBI Acc Number NP_000468.1
Standard Range Refer To Manual
Product Format In Solution
Specimen/Volumes Refer To Manual
Application ELISA
Reconstitution and Storage Store as indicated in product manual.
Reactivity Mouse
Kit Size 96 well
Kit Duration 70 Minutes
Datasheets/Manuals Printable datasheet for GWB-282C17
Storage Refer to kit instructions for component storage
Species Reactivity Mouse
Intended Use Research Use Only
Additional Information Intended Use: The Albumin test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Albumin in serum, plasma or urine of mice.
:: Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Mouse Albumin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-MOUSE ALBUMIN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Mouse albumin.
7. MOUSE ALBUMIN CALIBRATOR
One vial containing a lyophilized Mouse Albumin calibrator.