Isotype: IgG2a kappa
Characterization: To insure lot-to-lot consistency, each batch of product is tested for conformance with characteristics of a standard reference reagent by flow cytometry. Specificity: Mouse CD 45.2 (Leukocyte Common Antigen)
The CD45 antigen is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase, e.g. CD45 is able to dephosporylate the Src family of protein kinases.1, 2 This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling and Fc receptor stimulation. 1, 2 The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variants (CD45R; see companion products 1660, 1665, 1675, 1685 and 1820) and as allelic forms CD45.1/Ly-5.1 and CD45.2/Ly-5.2. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies where resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation. 3, 4 The monoclonal antibody 104-2 recognizes CD45 (Leukocyte Common Antigen) on all leukocytes of mouse strains expressing the CD45.2 allotype (e.g. A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). Working Dilution: Flow Cytometry: 0.3 ug/10^6 cells
Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. Function: Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits an dephosphorylates SKAP1 and FYN.
Catalytic Activity: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subunit: Interacts with SKAP1 (By similarity). Binds GANAB and PRKCSH.
Subcellular Location: Membrane; Single-pass type I membrane protein.
Developmental Stage: Expression is restricted to the hematopoietic compartment of development.
Domain: The first PTPase domain interacts with SKAP1 (By similarity).
Ptm: Heavily N- and O-glycosylated.
Similarity: Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Similarity: Contains 2 fibronectin type-III domains.
Similarity: Contains 2 tyrosine-protein phosphatase domains. 1. Okumura, M. and M. L. Thomas. 1995. Current Opin. Immunol. 7:312-319.
2. Thomas. M. L. 1995. Seminars in Immunol. 7:279-288.
3. Mardiney, M and H. L. Malech. 1996. Blood 87:4049-4056.
4. Vallera, D. A., et al. 1994. Transplantation 57:249-256.