Mouse IFN-gamma ELISA Kit (GWB-SKR076) (Aviva Cat. No. OKAG00076)

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Please see item number OKAG00076 to view product details and for ordering information.


GWB-SKR076

Aviva Cat. No.: OKAG00076

Description

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Murine IFN-gamma ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Murine IFN-gamma proteins within the range of 23-1500 pg/ml. The Murine IFN-gamma ELISA is capable of recognizing both recombinant and naturally produced Murine IFN-gamma proteins. The antigens listed below were tested at 50 ng/ml and exhibited 100% cross reactivity. • Rat IFN-gamma The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Human IFN-alpha, IFN-beta, IFN-gamma, IFN-?1, IFN-?2, IL-10, IL-18 • Murine IFN-?2, IL-10, IL-12, IL-18 • Rat IL-10

Additional Information

Name Mouse IFN-gamma ELISA Kit (GWB-SKR076)
Related Product Names Interferon gamma
Kit Range 23-1500 pg/mL
Sensitivity 23 pg/mL
Sample Type Cell lysates, sera and plasma
Kit Detection Colorimetric 450 nm
Gene Symbol IFNG
Gene Family IFN
Gene Fullname interferon gamma
Gene id 15978
Alias Symbols Ifg, IFN-g, IFN-gamma, Interferon gamma
Swissprot ID P01580
Protein Name Interferon gamma
Description of Target This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor w
Kit Principle The Aviva Murine IFN-γ ELISA Kit contains the components necessary for quantitative determination of natural or recombinant mIFN-γ concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Murine IFN-γ cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (100 ng)
Protein Accession Num NP_032363.1
Nucleotide Accession Num NM_008337.4
Assay Info Quantitative Colorimentric Sandwich ELISA
Standard Range 23-1500 pg/ml
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Specimen/Volumes Refer to Manual
Specificity The Murine IFN-γ ELISA is capable of recognizing both recombinant and naturally produced Murine IFN-γ proteins. The antigens listed below were tested at 50 ng/ml and exhibited 100% cross reactivity.
• Rat: IFN-γ
The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: IFN-α, IFN-β, IFN-γ, IFN-λ1, IFN-λ2, IL-10, IL-18
• Murine: IFN-λ2, IL-10, IL-12, IL-18
• Rat: IL-10
Application ELISA
GI Number 15978
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Mouse
Kit Size 12 x 8-Well Microstrips
Datasheets/Manuals Printable datasheet for GWB-SKR076
Storage 4C/6 Months
Species Reactivity Mouse
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only