Mouse IL-27 ELISA Kit (GWB-SKR186) (Aviva Cat. No. OKAG00186)

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Please see item number OKAG00186 to view product details and for ordering information.


GWB-SKR186

Aviva Cat. No.: OKAG00186

Description

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Murine IL-27 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Murine IL-27 proteins within the range of 16-1000 pg/ml. The Murine IL-27 ELISA is capable of recognizing both recombinant and naturally produced Murine IL-27 proteins. The antigens listed below were tested at 100 ng/ml and did not exhibit significant cross reactivity or interference. • Human IL-12 p40 • Murine IL-6, IL-6R, IL-12, IL-12 p35, IL-12 p40, IL-23, IL-23 p19, TCCR/Fc Chimera

Additional Information

Name Mouse IL-27 ELISA Kit (GWB-SKR186)
Related Product Names Interleukin-27 subunit alpha/beta, p28, Epstein-Barr virus-induced gene 3 protein
Kit Range 16-1,000 pg/mL
Sensitivity 16 pg/mL
Sample Type Cell lysates, sera and plasma
Kit Detection Colorimetric 450 nm
Gene Symbol IL27
Gene Fullname interleukin 27
Gene id 246779; 50498
Alias Symbols IL-27, Il27a, IL27-A, IL-27-A, IL-27p28, IL-27 subunit alpha, Il30, Interleukin-27 subunit alpha, p28
Swissprot ID Q8K3I6
Protein Name Interleukin-27 subunit alpha
Description of Target Associates with EBI3 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotox
Kit Principle The Aviva Murine IL-27 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant mIL-27 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Murine IL-27 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (2 ng)
Protein Accession Num NP_663611.1
Nucleotide Accession Num NM_145636.1
Assay Info Quantitative Colorimentric Sandwich ELISA
Standard Range 16-1000 pg/ml
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Specimen/Volumes Refer to Manual
Specificity The Murine IL-27 ELISA is capable of recognizing both recombinant and naturally produced Murine IL-27 proteins. The antigens listed below were tested at 100 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: IL-12 p40
• Murine: IL-6, IL-6R, IL-12, IL-12 p35, IL-12 p40, IL-23, IL-23 p19, TCCR/Fc Chimera
Application ELISA
GI Number 246779/50498
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Mouse
Kit Size 12 x 8-Well Microstrips
Datasheets/Manuals Printable datasheet for GWB-SKR186
Storage 4C/6 Months
Species Reactivity Mouse
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only