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96 Wells
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Additional Information

Name MTNR1B ELISA Kit (Human) (GWB-KBBRV4)
Description of Target This gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This gene product is an integral membrane protein that is a G-protein coupled, 7-transmembrane receptor. It is found primarily
Kit Range 0.156-10 ng/mL
Sensitivity < 0.058 ng/mL
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol MTNR1B
Gene id 4544
Alias Symbols FGQTL2, Mel-1B-R, MEL-1B-R, Mel1b receptor, Melatonin receptor type 1B, MT2
Swissprot ID P49286
Protein Name Melatonin receptor type 1B
Kit Principle Genway Biotech MTNR1B ELISA Kit (Human) (GWB-KBBRV4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MTNR1B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MTNR1B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MTNR1B captured in the well.
Kit Component
Anti-MTNR1B Microplate96 Wells (12 x 8 Well Strips)
MTNR1B Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X MTNR1B Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_005950.1
Nucleotide Accession Num NM_005959.3
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBRV4
Species Reactivity Human