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NDUFS1 ELISA Kit (Human) (GWB-KBBIM3)

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GWB-KBBIM3

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Additional Information

Name NDUFS1 ELISA Kit (Human) (GWB-KBBIM3)
Description of Target Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.127 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol NDUFS1
Gene id 4719
Alias Symbols CI-75k, CI-75kD, CI-75Kd, Complex I-75kD, MGC26839, NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial, PRO1304
Swissprot ID P28331
Protein Name NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
Kit Principle Genway Biotech NDUFS1 ELISA Kit (Human) (GWB-KBBIM3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NDUFS1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NDUFS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NDUFS1 captured in the well.
Kit Component
ComponentAmount
Anti-POT1 Microplate96 Wells (12 x 8 Well Strips)
POT1 Lyophilized Standard2 x 20 ng
100X Biotinylated POT1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001186910
Nucleotide Accession Num NM_001199981
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBIM3
Species Reactivity Human