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Additional Information

Name NET1 ELISA Kit (Human) (GWB-KBBMS9)
Description of Target Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.125 ng/mL
Sample Type tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol NET1
Gene id 10276
Alias Symbols ARHGEF8, NET1A, Neuroepithelial cell-transforming gene 1 protein, Proto-oncogene p65 Net1, Rho guanine nucleotide exchange factor 8
Swissprot ID Q7Z628
Protein Name Neuroepithelial cell-transforming gene 1 protein
Kit Principle Genway Biotech NET1 ELISA Kit (Human) (GWB-KBBMS9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NET1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NET1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NET1 captured in the well.
Kit Component
Anti-NET1 Microplate96 Wells (12 x 8 Well Strips)
NET1 Lyophilized Standard2 x 20 ng
100X Biotinylated NET1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001040625
Nucleotide Accession Num NM_001047160
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBMS9
Species Reactivity Human