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Additional Information

Name OAS1 ELISA Kit (Human) (GWB-KBBGO9)
Description of Target Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. S
Kit Range 4.69 - 300 ng/mL
Sensitivity < 1.9 ng/mL
Sample Type serum, plasma and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol OAS1
Gene id 4938
Alias Symbols (2-5')oligo(A) synthase 1, 2'-5'-oligoadenylate synthase 1, 2-5A synthase 1, E18/E16, IFI-4, OIAS, OIASI, p46/p42 OAS
Swissprot ID P00973
Protein Name 2'-5'-oligoadenylate synthase 1
Kit Principle Genway Biotech OAS1 ELISA Kit (Human) (GWB-KBBGO9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for OAS1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for OAS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample OAS1 captured in the well.
Kit Component
Anti-OAS1 Microplate96 Wells (12 x 8 Well Strips)
OAS1 Lyophilized Standard2 x 300 ng
100X Biotinylated OAS1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001027581
Nucleotide Accession Num NM_001032409
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)87-101%96-105%81-103%98-105%
EDTA Plasma (n=5)85-92%87-96%93-101%81-94%
Heparin Plasma (n=5)94-103%85-93%93-101%85-97%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)92-9995
EDTA Plasma (n=5)78-9286
Heparin Plasma (n=5)92-10196
Datasheets/Manuals Printable datasheet for GWB-KBBGO9
Species Reactivity Human