OXCT1 ELISA Kit (Human) (GWB-KBBWS2) (Aviva Cat. No. OKCD01034)

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Aviva Cat. No.: OKCD01034

Additional Information

Name OXCT1 ELISA Kit (Human) (GWB-KBBWS2)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.124 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol OXCT1
Gene id 5019
Alias Symbols 3-oxoacid-CoA transferase 1, OXCT, SCOT, SCOT-s, Somatic-type succinyl-CoA:3-oxoacid-CoA-transferase, Succinyl-CoA:3-ketoacid-coenzyme A transferase 1, mitochondrial
Swissprot ID P55809
Protein Name succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial
Description of Target Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.
Kit Principle Genway Biotech OXCT1 ELISA Kit (Human) (GWB-KBBWS2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for OXCT1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for OXCT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample OXCT1 captured in the well.
Kit Component
Anti-NFRKB Microplate96 Wells (12 x 8 Well Strips)
NFRKB Lyophilized Standard2 x 20 ng
100X Biotinylated NFRKB Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_000427
Nucleotide Accession Num NM_000436
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBWS2
Species Reactivity Human