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P4HA3 ELISA Kit (Human) (GWB-KBBPS1)

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GWB-KBBPS1

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Additional Information

Name P4HA3 ELISA Kit (Human) (GWB-KBBPS1)
Description of Target Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.066 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol P4HA3
Gene id 283208
Alias Symbols 4-PH alpha-3, C-P4Halpha(III), Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3, Prolyl 4-hydroxylase subunit alpha-3, UNQ711/PRO1374
Swissprot ID Q7Z4N8
Protein Name prolyl 4-hydroxylase subunit alpha-3
Kit Principle Genway Biotech P4HA3 ELISA Kit (Human) (GWB-KBBPS1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for P4HA3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for P4HA3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample P4HA3 captured in the well.
Kit Component
ComponentAmount
Anti-TDO2 Microplate96 Wells (12 x 8 Well Strips)
TDO2 Lyophilized Standard2 x 10 ng
100X Biotinylated TDO2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001275677
Nucleotide Accession Num NM_001288748
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBPS1
Species Reactivity Human