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P4Ha3 ELISA Kit (Human) (GWB-KBBTU3)

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96 Wells
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Additional Information

Name P4Ha3 ELISA Kit (Human) (GWB-KBBTU3)
Description of Target This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part o
Kit Range 0.156-10 ng/mL
Sensitivity < 0.066 ng/mL
Sample Type Serum, plasma, tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol P4Ha3
Gene id 283208
Alias Symbols 4-PH alpha-3, C-P4Halpha(III), Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3, Prolyl 4-hydroxylase subunit alpha-3, UNQ711/PRO1374
Swissprot ID Q7Z4N8
Protein Name Prolyl 4-hydroxylase subunit alpha-3
Kit Principle Genway Biotech P4Ha3 ELISA Kit (Human) (GWB-KBBTU3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for P4Ha3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for P4Ha3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample P4Ha3 captured in the well.
Kit Component
Anti-P4Ha3 Microplate96 Wells (12 x 8 Well Strips)
P4Ha3 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X P4Ha3 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001275677.1
Nucleotide Accession Num NM_001288748.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBTU3
Species Reactivity Human