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Pgp ELISA Kit (Rat) (GWB-KBBTL9)

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GWB-KBBTL9

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Additional Information

Name Pgp ELISA Kit (Rat) (GWB-KBBTL9)
Description of Target Glycerol-3-phosphate phosphatase hydrolyzing glycerol-3-phosphate into glycerol.
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.056 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol PGP
Gene id 287115
Alias Symbols Pgp, Glycerol-3-phosphate phosphatase
Swissprot ID D3ZDK7
Protein Name Glycerol-3-phosphate phosphatase
Kit Principle Genway Biotech Pgp ELISA Kit (Rat) (GWB-KBBTL9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pgp has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pgp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pgp captured in the well.
Kit Component
ComponentAmount
Anti-Pgp Microplate96 Wells (12 x 8 Well Strips)
Pgp Lyophilized Standard2 x 10 ng
100X Biotinylated Pgp Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001162623
Nucleotide Accession Num NM_001169152
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBTL9
Species Reactivity Rat