PLUNC ELISA Kit (Human) (GWB-KBBFD3) (Aviva Cat. No. OKDD00474)

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Please see item number OKDD00474 to view product details and for ordering information.


Aviva Cat. No.: OKDD00474

Additional Information

Kit Range 0.156-10 ng/mL
Sensitivity < 0.055 ng/mL
Sample Type Serum, plasma, tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol PLUNC
Gene Fullname BPI fold containing family A member 1
Gene id 51297
Alias Symbols bA49G10.5, LPLUNC3, Lung-specific protein X, LUNX, NASG, Nasopharyngeal carcinoma-related protein, Palate lung and nasal epithelium clone protein, Protein Plunc, Secretory protein in upper respiratory tracts, SPLUNC1, SPURT, Tracheal epithelium-enriched p
Swissprot ID Q9NP55
Protein Name BPI fold-containing family A member 1
Kit Principle Genway Biotech PLUNC ELISA Kit (Human) (GWB-KBBFD3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PLUNC has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PLUNC is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PLUNC captured in the well.
Kit Component
Anti-PLUNC Microplate96 Wells (12 x 8 Well Strips)
PLUNC Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X PLUNC Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001230122.1
Nucleotide Accession Num NM_001243193.1
Assay Info Quantitative Sandwich ELISA
Molecular Weight 27 kDa
Specificity This assay has high sensitivity and excellent specificity for detection of PLUNC. No significant cross-reactivity or interference between PLUNC and analogues was observed.
Reconstitution and Storage Store as indicated in product manual.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBFD3
Species Reactivity Human