PicoProbeAcetyl-CoA Assay Kit (GWB-AXR197)

GWB-AXR197

100 assays
$472.50

Description

Description: Acetyl CoA is a central molecule of metabolism. It carries acetate, used in the build-up and breakdown of larger molecules. Acetyl CoA is key in synthetic pathways leading to sesquiterpenes, precursors to cholesterol and other sterols, flavenoids and other polyketides, polyenes and long-chain fatty acids. It is the source of the acetyl group used in histone acetylation. The acetyl group is also incorporated into a variety of other molecules such as acetylcholine, melatonin, heme and TCA cycle intermediates. GenWay has developed a highly sensitive assay for determining Acetyl CoA level in a variety of biological samples. In the assay, free CoA is quenched then Acetyl CoA is converted to CoA. The CoA is reacted to form NADH which interacts with PicoProbe to generate fluorescence (Ex=535/Em=587 nm). The assay can detect 10 to 1000 pmol of Acetyl CoA (with detection limit ~0.4 µM) in a variety of samples.

Summary:

- Detection method- Fluorescence (Ex/Em 535/587 nm)

- Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

- Species reactivity- Mammalian

- Applications- The assay can detect 10 to 1000 pmol of Acetyl CoA (with detection limit ~0.4 µM) in a variety of samples.

Kit components:

- Acetyl CoA Assay Buffer

- PicoProbe

- Conversion Enzyme

- Acetyl CoA Enzyme Mix

- Acetyl CoA Substrate Mix

- CoA Quencher

- Quench Remover

- Acetyl CoA Standard (10 µmol)

Features and Benefits:

- Very simple procedure

- Convenient

- GenWay has developed a high sensitivity assay for Acetyl CoA level in a variety of biological samples. In the assay, free CoA is quenched then Acetyl CoA is converted to CoA. The CoA is reacted to form NADH which interacts with PicoProbe to generate fluorescence.

Shipping Conditions: Gel pack

Additional Information

Name PicoProbeAcetyl-CoA Assay Kit (GWB-AXR197)
Related Product Names Acetyl-CoA, Acetyl CoA, Acetyl coenzymeA, Acetyl Coenzyme A
Format Assay Kit
Storage -20C
Datasheets/Manuals Printable datasheet for GWB-AXR197
Intended Use Research Use Only