AKT3, Active

GWB-53675A

10-782-55011

$0.00

Description

Application Note Kinase assay Recombinant full-length human AKT3 was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
Catalytic Activity ATP + a protein = ADP + a phosphoprotein.
Similarity Belongs to the Ser/Thr protein kinase family.

Contains 1 PH domain.
Storage, Stability and Shipping Store product at -70C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications Kinase Assay, Western Blot
Molecular Weight ~85 kDa
Gene Aliases PKBG; PRKBG; STK-2; RAC-gamma; RAC-PK-gamma; DKFZP434N0250
Scientific Background AKT 3 or Protein Kinase B γ (PKBγ) is a serine/threonine kinase that is a member of the AKT family. AKT 3 is activated in cells exposed to diverse stimuli such as hormones, growth factors, and extracellular matrix components (1). AKT 3 phosphorylates and regulates the function of many cellular proteins involved in processes that include cellular metabolism, survival/apoptosis, and proliferation. Recent evidence indicates that AKT 3 is frequently overexpressed in many types of human cancers including breast and prostate (2).
Summary The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described.
References
  1. Pedrero,J.M., Carracedo,D.G., Pinto,C.M., Zapatero,A.H., Rodrigo,J.P., Nieto,C.S. and Gonzalez,M.V.
    Frequent genetic and biochemical alterations of the PI 3-K/AKT/PTEN pathway in head and neck squamous cell carcinoma
  2. Kanekura,K., Hashimoto,Y., Kita,Y., Sasabe,J., Aiso,S., Nishimoto,I. and Matsuoka,M.
    A Rac1/phosphatidylinositol 3-kinase/Akt3 anti-apoptotic pathway, triggered by AlsinLF, the product of the ALS2 gene, antagonizes Cu/Zn-superoxide dismutase (SOD1) mutant-induced motoneuronal cell death
  3. Taniyama,Y., Ito,M., Sato,K., Kuester,C., Veit,K., Tremp,G., Liao,R., Colucci,W.S., Ivashchenko,Y., Walsh,K. and Shiojima,I.
    Akt3 overexpression in the heart results in progression from adaptive to maladaptive hypertrophy
  4. Cicenas,J., Urban,P., Vuaroqueaux,V., Labuhn,M., Kung,W., Wight,E., Mayhew,M., Eppenberger,U. and Eppenberger-Castori,S.
    Increased level of phosphorylated akt measured by chemiluminescence-linked immunosorbent assay is a predictor of poor prognosis in primary breast cancer overexpressing ErbB-2
  5. Stahl,J.M., Sharma,A., Cheung,M., Zimmerman,M., Cheng,J.Q., Bosenberg,M.W., Kester,M., Sandirasegarane,L. and Robertson,G.P.
    Deregulated Akt3 activity promotes development of malignant melanoma
  6. Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., et al.
    Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.

Additional Information

Name AKT3, Active
Availability Discontinued
Related Product Names AKT3 proteinAKT3
Swissprot ID Q56A86
NCBI Acc Number NP_005456.1
Molecular Weight 0.5
Source Insect Cells
GI Number 4885549
Stability 1 year at -20 degree C.
Sequence Length 479
Storage Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
Datasheets/Manuals Printable datasheet for GWB-53675A
Intended Use Research Use Only