AKT2, Active (GWB-84F74D)





Application Note: Kinase assay Recombinant full-length human AKT2 was expressed by baculovirus in Sf9 insect cells using a N-terminal His tag.

Function: General protein kinase capable of phosphorylating several known proteins.

Catalytic Activity: ATP + a protein = ADP + a phosphoprotein.

Tissue Specificity: In all human cell types so far analyzed.

Disease: Alterations of AKT2 may contribute to the pathogenesis of ovarian carcinomas.

Similarity: Belongs to the Ser/Thr protein kinase family. RAC subfamily.

Similarity: Contains 1 PH domain.

Similarity: Contains 1 protein kinase domain.

Summary: AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins. [1] Pu,P., Kang,C., Li,J., Jiang,H. and Cheng,J., et al.
The effects of antisense AKT2 RNA on the inhibition of malignant glioma cell growth in vitro and in vivo
[2] Irie,H.Y., Pearline,R.V., Grueneberg,D., Hsia,M., Ravichandran,P., Kothari,N., Natesan,S. and Brugge,J.S.
Distinct roles of Akt1 and Akt2 in regulating cell migration and epithelial-mesenchymal transition
[3] Baer,K., Lisinski,I., Gompert,M., Stuhlmann,D., Schmolz,K., Klein,H.W. and Al-Hasani,H.
Activation of a GST-tagged AKT2/PKBbeta
[4] Shiue,H., Musch,M.W., Wang,Y., Chang,E.B. and Turner,J.R., et al.
Akt2 phosphorylates ezrin to trigger NHE3 translocation and activation
[5] Cicenas,J., Urban,P., Vuaroqueaux,V., Labuhn,M., Kung,W., Wight,E., Mayhew,M., Eppenberger,U. and Eppenberger-Castori,S.
Increased level of phosphorylated akt measured by chemiluminescence-linked immunosorbent assay is a predictor of poor prognosis in primary breast cancer overexpressing ErbB-2
[6] Jones P.F., Jakubowicz T., Hemmings B.A.
Molecular cloning of a second form of rac protein kinase.
[7] Cheng J.Q., Godwin A.K., Bellacosa A., Taguchi T., Franke T.F., Hamilton T.C., Tsichlis P.N., Testa J.R.
AKT2, a putative oncogene encoding a member of a subfamily of protein-serine/threonine kinases, is amplified in human ovarian carcinomas.
[8] Rieder M.J., Livingston R.J., Braun A.C., Montoya M.A., Chung M.-W., Miyamoto K.E., Nguyen C.P., Nguyen D.A., Poel C.L., Robertson P.D., et al.
NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu).

Additional Information

Name AKT2, Active (GWB-84F74D)
Related Product Names EC; RAC-PK-beta; Protein kinase Akt-2; Protein kinase B, beta; PKB beta RAC-beta serine/threonine-protein kinaseAKT2
Swissprot ID P31751
NCBI Acc Number NP_001617.1
Molecular Weight 0.5
Source Insect Cells
GI Number 4502023
Stability 1 year at -20 degree C.
Sequence Length 481
Storage Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
Datasheets/Manuals Printable datasheet for GWB-84F74D
Intended Use Research Use Only