Anti- Calcium/calmodulin-dependent protein kinase type II alpha chain Antibody (GWB-93B246)

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Similarity: Contains 1 protein kinase domain.

Function: CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.

Additional Information

Name Anti- Calcium/calmodulin-dependent protein kinase type II alpha chain Antibody (GWB-93B246)
Related Product Names EC; CaM-kinase II alpha chain; CaM kinase II alpha subunit; CaMK-II subunit alpha PKCCD; PK2CDDCalcium/calmodulin-dependent protein kinase type II alpha chainCamk2a
Description of Target Ca2+/Calmodulin-Dependent Protein Kinase II (CaM Kinase II) is a
multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular
responses to a wide variety of intercellular signals (Kennedy, 1998; Schulman and Hanson,
Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr305 found in rat brain CaM Kinase II.
Gene Symbol CAMK2A/B
Gene id 25400
Swissprot ID P11275
Protein Name Calcium/calmodulin-dependent protein kinase type II subunit alpha
Protein Accession Num NP_037052.1
Host Rabbit
Homology Cow; Dog; Human; Mouse; Rat; Zebrafish
NCBI Acc Number NP_037052.1
Product Format Liquid. Neat serum. Adequate amount of material to conduct 10-mini Western blots.
Molecular Weight ~50kDa Alpha & ~60kDa Beta
Purification Unpurified neat serum.
Specificity Specific for the ~50k alpha -CaM Kinase II and the ~60k beta -CaM Kinase II
proteins phosphorylated at Thr305. Immunolabeling is blocked by the phosphopeptide used as
antigen but not by the corresponding dephosphopeptide.
Application WB
GI Number 6978593
Clonality Serum
Stability 6-12 months at -20 degree C.
Reconstitution and Storage For long term storage -20C is recommended. Stable at -20C for at least 1 year.
Reactivity Cow, chicken, human, mouse, rat, xenopus
Sequence Length 478
Application Info The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, chicken, canine, human, mouse, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen. The antibody should also recognize the delta CaM kinase II subunit based on sequence homology with the antigen.
Western blot of rat brain lysate showing specific immunolabeling of the ~50k alpha - and the ~60k beta -CaM Kinase II phosphorylated at Thr305 (Control). The immunolabeling is blocked by the phosphopeptide used as the antigen (Peptide) but not by the corresponding dephosphopeptide (not shown).
Chrom Location 18q11
Storage 4 degree C for short term (weeks) and -20 degree C for long term. Avoid frequent freeze and thaw.
Datasheets/Manuals Printable datasheet for GWB-93B246
Species Reactivity Cow, Dog, Human, Mouse, Rat, Zebrafish
Intended Use Research Use Only
Key Reference Elgersma Y, Fedorov NB, Ikonen S, Choi ES, Elgersma M, Carvalho OM, Giese KP, Silva AJ (2002) Inhibitory
autophosphorylation of CaMKII controls PSD association, plasticity, and learning. Neuron 36:493-505.
Gleason MR, Higashijima S, Dallman J, Liu K, Mandel G, Fetcho JR (2003) Translocation of CaM kinase II to
synaptic sites in vivo. Nature Neurosci 6:217-218.
Hudmon A, Schulman H (2002) Neuronal Ca2+/calmodulin-dependent protein kinase II: The role of structure and
autoregulation in cellular function. Annu Rev Biochem 71:473-510.
Kennedy MB (1998) Signal transduction molecules at the glutamatergic postsynaptic membrane. Brain Res Rev
Meng FJ, Guo J, Zhang QG, Song B, Zhang GY (2003) Autophosphorylated calcium/calmodulin-dependent protein
kinase IIa (CaMKIIa) reversibly targets to and phosphorylates N-methyl-D-aspartate receptor subunit 2B (NR2B)
in cerebral ischemia and reperfusion in hippocampus of rats. Brain Res 967:161-169.
Picciotto MR, Czernik AJ, Nairn AC (1993) Calcium/calmodulin-dependent protein kinase I. cDNA cloning and
identification of autophosphorylation site. J Biol Chem 268:26512-26521.
Schulman H, Hanson PI (1993) Multifunctional Ca2+/calmodulin-dependent protein kinase. Neurochem Res 18:65-77.
Soderling TR (2000) CaM-kinases: modulators of synaptic plasticity. Curr Opin Neurobiol 10:375-380.
Haiqin Lu, Hung-Tat Leung, Ning Wang, William L. Pak, and Bih-Hwa Shieh (2009) Role of Ca2+/Calmodulindependent
Protein Kinase II in Drosophila Photoreceptors J. Biol. Chem., 284: 11100 - 11109.
Rebekah S. Vest, Kurtis D. Davies, Heather O'Leary, J. David Port, and K. Ulrich Bayer (2007) Dual Mechanism of a
Natural CaMKII Inhibitor. Mol. Biol. Cell, 18: 5024 - 5033.
Matthew Townsend, Tapan Mehta, and Dennis J. Selkoe (2007) Soluble A beta Inhibits Specific Signal Transduction
Cascades Common to the Insulin Receptor Pathway. J. Biol. Chem., 282: 33305 - 33312
Additional Information Packaging :
Neat serum. Adequate amount of material to conduct 10-mini Western blots.