CAMK4, Active (GWB-80078B)





Application Note: Kinase assay Recombinant full-length human CAMK4 was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Function: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory (By similarity).

Catalytic Activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme Regulation: Activated by Ca(2+)/calmodulin. Binding of calmodulin may releave intrasteric autoinhibition. Must be phosphorylated to be maximally active. Phosphorylated by CAMKK1 or CAMKK2. Autophosphorylation of the N-terminus is required for full activation. In part, activity is independent on Ca(2+)/calmodulin and autophosphorylation of Ser-336 allows to switch to a Ca(2+)/calmodulin-independent state (By similarity). Probably inactivated by serine/threonine protein phosphatase 2A.

Subunit: Monomer (By similarity). Interacts with serine/threonine protein phosphatase 2A catalytic subunit, PPP2CA or PPP2CB. The interaction with PP2CA or PP2CB is mutually exclusive with binding to Ca(2+)/calmodulin.

Subcellular Location: Cytoplasm (By similarity). Nucleus (By similarity). Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix (By similarity).

Tissue Specificity: Expressed in epithelial ovarian cancer tissue.

Ptm: Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200.

Similarity: Belongs to the Ser/Thr protein kinase family. CaMK subfamily.

Similarity: Contains 1 protein kinase domain.

Summary: The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. [1] Marin-Briggiler,C.I., Jha,K.N., Chertihin,O., Buffone,M.G., Herr,J.C., Vazquez-Levin,M.H. and Visconti,P.E.
Evidence of the presence of calcium/calmodulin-dependent protein kinase IV in human sperm and its involvement in motility regulation
[2] Juang,Y.T., Wang,Y., Solomou,E.E., Li,Y., Mawrin,C., Tenbrock,K., Kyttaris,V.C. and Tsokos,G.C.
Systemic lupus erythematosus serum IgG increases CREM binding to the IL-2 promoter and suppresses IL-2 production through CaMKIV
[3] Ishida,A., Tada,Y., Nimura,T., Sueyoshi,N., Katoh,T., Takeuchi,M., Fujisawa,H., Taniguchi,T. and Kameshita,I.
Identification of major Ca(2+)/calmodulin-dependent protein kinase phosphatase-binding proteins in brain: biochemical analysis of the interaction
[4] Cuschieri,J., Bulger,E., Garcia,I., Jelacic,S. and Maier,R.V., et al.
Calcium/calmodulin-dependent kinase II is required for platelet-activating factor priming
[5] Yamamori,E., Asai,M., Yoshida,M., Takano,K., Itoi,K., Oiso,Y. and Iwasaki,Y.
Calcium/calmodulin kinase IV pathway is involved in the transcriptional regulation of the corticotropin-releasing hormone gene promoter in neuronal cells
[6] Kitani T., Okuno S., Fujisawa H.
cDNA cloning and expression of human calmodulin-dependent protein kinase IV.
[7] Bland M.M., Monroe R.S., Ohmstede C.A.
The cDNA sequence and characterization of the Ca2+/calmodulin-dependent protein kinase-Gr from human brain and thymus.
[8] Mosialos G., Hanissian S.H., Jawahar S., Vara L., Kieff E., Chatila T.A.
A Ca2+/calmodulin-dependent protein kinase, CaM kinase-Gr, expressed after transformation of primary human B lymphocytes by Epstein-Barr virus (EBV) is induced by the EBV oncogene LMP1.
[9] Chatila T., Anderson K.A., Ho N., Means A.R.
A unique phosphorylation-dependent mechanism for the activation of Ca2+/calmodulin-dependent protein kinase type IV/GR.
[10] Melander Gradin H., Marklund U., Larsson N., Chatila T.A., Gullberg M.
Regulation of microtubule dynamics by Ca2+/calmodulin-dependent kinase IV/Gr-dependent phosphorylation of oncoprotein 18.

Additional Information

Name CAMK4, Active (GWB-80078B)
Related Product Names EC; CAM kinase-GR; CaMK IV Calcium/calmodulin-dependent protein kinase type IVCAMK4
Swissprot ID Q16566
NCBI Acc Number NP_001735.1
Molecular Weight 0.5
Source Insect Cells
GI Number 4502557
Stability 1 year at -20 degree C.
Sequence Length 473
Storage Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
Datasheets/Manuals Printable datasheet for GWB-80078B
Intended Use Research Use Only