Anti- IKK -alpha (Phospho-Thr23) Antibody

GWB-2F07A4

18-785-210222

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Description

Binding Site: Thr23

Form of Antibody: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. WB Dilution: 1:500~1:1000

IHC Dilution: 1:50~1:100

Specificity/Sensitivity: IKK? (phospho-Thr23) antibody detects endogenous levels of IKK? only when phosphorylated at threonine 23.

Applications: WB: 1:500~1:1000 IHC: 1:50~1:100 Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human IKK-alpha around the phosphorylation site of threonine 23 (L-G-TP-G-G). Function: Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3. Phosphorylates \'Ser-10\' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.

Catalytic Activity: ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].

Enzyme Regulation: Activated when phosphorylated and inactivated when dephosphorylated.

Subunit: Preferentially found as a heterodimer with IKK-beta but also as an homodimer. Directly interacts with IKK-gamma/NEMO and TRPC4AP (By similarity). Heterodimers form the active complex. The tripartite complex can also bind to MAP3K14/NIK, MEKK1, IKAP and IKB-alpha-p65-p50 complex. A weak interaction with TRAF2 cannot be excluded. Part of a complex composed of NCOA2, NCOA3, IKKB, IKBKG and CREBBP. Interacts with NALP2. May interact with MAVS/IPS1.

Subcellular Location: Cytoplasm. Nucleus. Note=Shuttles between the cytoplasm and the nucleus.

Tissue Specificity: Widely expressed.

Ptm: Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.

Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. IkappaB kinase subfamily.

Similarity: Contains 1 protein kinase domain. [1] Regnier C.H., Song H.Y., Gao X., Goeddel D.V., Cao Z., Rothe M.
Identification and characterization of an IkappaB kinase.
[2] DiDonato J.A., Hayakawa M., Rothwarf D.M., Zandi E., Karin M.
A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB.
[3] Mercurio F., Zhu H., Murray B.W., Shevchenko A., Bennett B.L., Li J.W., Young D.B., Barbosa M., Mann M., Manning A., et al.
IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation.
[4] Hu M.C.-T., Wang Y.-P.
IkappaB kinase-alpha and -beta genes are coexpressed in adult and embryonic tissues but localized to different human chromosomes.
[5] Rieder M.J., Daniels R.L., da Ponte S.H., Hastings N.C., Ahearn M.O., Rajkumar N., Yi Q., Nickerson D.A.
SeattleSNPs. NHLBI HL66682 program for genomic applications, UW-FHCRC, Seattle, WA (URL: http://pga.gs.washington.edu).
[6] Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., et al.
The DNA sequence and comparative analysis of human chromosome 10.
[7] Connelly M.A., Marcu K.B.
CHUK, a new member of the helix-loop-helix and leucine zipper families of interacting proteins, contains a serine-threonine kinase catalytic domain.
[8] Ling L., Cao Z., Goeddel D.V.
NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176.
[9] Ozes O.N., Mayo L.D., Gustin J.A., Pfeffer S.R., Pfeffer L.M., Donner D.B.
NF-kappaB activation by tumour necrosis factor requires the Akt serine-threonine kinase.
[10] Delhase M., Hayakawa M., Chen Y., Karin M.
Positive and negative regulation of IkappaB kinase activity through IKKbeta subunit phosphorylation.

Additional Information

Name Anti- IKK -alpha (Phospho-Thr23) Antibody
Related Product Names EC 2.7.11.10; I kappa-B kinase alpha; IkBKA; IKK-alpha; IKK-A; IkappaB kinase; I-kappa-B kinase 1; IKK1; Conserved helix-loop-helix ubiquitous kinase; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA IKKA; TCF16IKK -alpha (Phospho-Thr23)IKKa
Swissprot ID O15111
Molecular Weight 84654
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation
Source Rabbit
Applications IHC, WB
Clonality Polyclonal
Format In PBS, 0.02% sodium azide and 50% glycerol.
Stability 1 year at -20C
Reactivity Human, Mouse, Rat
Storage Store at -20C/1 year
Datasheets/Manuals Printable datasheet for GWB-2F07A4
Intended Use Research Use Only