Mouse Monoclonal Antibody to JAK3 (GWB-7D577B)

Description: JAK3, Janus kinase 3. It is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease).

Isotype: Mouse IgG1

Clone: 5H2

Immunogen: Purified recombinant fragment of human JAK3 expressed in E. Coli.

Formulation: Ascitic fluid containing 0.03% sodium azide.

Application: Western Bloting: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test. 1. J Biol Chem. 1995 Oct 20;270(42):25028-36.
2. Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6374-8.
3. Leuk Lymphoma. 2002 Dec;43(12):2355-62.