C-JUN Protein (GWB-8D9488)

GWB-8D9488

10-663-45566

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Description

Solubility: It is recommended to centrifuge the vial prior to opening in order to bring the contents to the bottom. The reconstitution of the lyophilized c-Jun is recommended in 40mM Tris, pH 7.5, to a concentration of 0.2-1.0 mg/ml. C-JUN amino acids 1-81 produced in E.coli, is a non-glycosylated, polypeptide chain having a molecular mass of 52 kDa.

Similarity: Contains 1 bZIP domain.

Function: Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.

Additional Information

Name C-JUN Protein (GWB-8D9488)
Availability In Stock
Related Product Names Transcription factor AP-1, Activator protein 1, AP1, Proto-oncogene c-jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, c-Jun.; Activator protein 1; AP1; Proto-oncogene c-jun; V-jun avian sarcoma virus 17 oncogene homolog; p39 C-JUN HumanJUN
Gene Symbol C-JUN
Gene id 3725
Alias Symbols Transcription factor AP-1, Activator protein 1, AP1, Proto-oncogene c-jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, c-Jun, C-JUN Human, C-JUN Human Recombinant
Swissprot ID P05412
Protein Name Transcription factor AP-1
Description of Target Recombinant C-JUN
Protein Accession Num NP_002219.1
Host E. Coli
NCBI Acc Number NP_002219.1
Product Format C-JUN is supplied as lyophilized powder containing no additives. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity Greater than 95% as determined by SDS-PAGE.
Format C-JUN is supplied as lyophilized powder containing no additives.
Reconstitution and Storage Store at 4C if entire vial will be used within 1-2 weeks. Store, frozen at -20C for longer periods of time. Avoid multiple freeze-thaw cycles.
Storage Store at 4 C if entire vial will be used within 1-2 weeks. Store, frozen at -20 C for longer periods of time. Avoid multiple freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for GWB-8D9488
Intended Use Research Use Only
Additional Information Solubility: It is recommended to centrifuge the vial prior to opening in order to bring the contents to the bottom. The reconstitution of the lyophilized c-Jun is recommended in 40mM Tris, pH 7.5, to a concentration of 0.2-1.0 mg/ml.
:: Biological Activity: C-Jun is phosphorylatable in vitro, using either recombinant active JNK1 or JNK2, or with JNK immunoprecipitated from stimulated cells. This phosphorylation can be monitored by Western blot analysis using an antibody directed to c-Jun [pS73], in conjunction with chemiluminescence detection methods. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application.
Product Note: Kinase activity may vary depending on the substrate and reaction conditions.
:: Product Introduction: C-JUN is a gene which, in combination with c-Fos, forms the AP-1early response transcription factor. It is activated by the JNKpathway.This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
Product Description: C-JUN amino acids 1-81 produced in E.coli, is a non-glycosylated, polypeptide chain having a molecular mass of 52 kDa.C-JUN is a maltose binding protein (MBP) fusion protein with an amino-terminal polyhistidine tag and purified by proprietary chromatographic techniques.