JNK2a-1 Human (GWB-7B0204)

GWB-7B0204

10-663-45542

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Description

Similarity: Contains 1 protein kinase domain.

Function: JNK2 isoforms display different binding patterns alpha-1 and alpha-2 preferentially bind to c-Jun whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it.

Additional Information

Name JNK2a-1 Human (GWB-7B0204)
Availability In Stock
Related Product Names Mitogen-activated protein kinase 9, EC 2.7.11.24, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2, JNK-55, JNK2, JNK2A, JNK2B, PRKM9, JNK2BETA, p54aSAPK, JNK2ALPHA, MAPK9, JUN N-terminal Kinase 2 alpha 1, JNK2a-1.; EC 2.7.11.24; Stress-act
Gene Symbol JNK2a-1
Gene id 5601
Alias Symbols Mitogen-activated protein kinase 9, EC 2.7.11.24, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2, JNK-55, JNK2, JNK2A, JNK2B, PRKM9, JNK2BETA, p54aSAPK, JNK2ALPHA, MAPK9, JUN N-terminal Kinase 2 alpha 1, JNK2a-1, JNK2a-1 Hu
Swissprot ID P45984-2
Protein Name Mitogen-activated protein kinase 9
Description of Target Recombinant Human Jun N-terminal kinase 2, alpha 1
Protein Accession Num NP_001128516.1
Host E. Coli
Peptide Sequence MSDSKCDSQF YSVQVADSTF TVLKRYQQLK PIGSGAQGIV CAAFDTVLGI NVAVKKLSRP FQNQTHAKRAYRELVLLKCV NHKNIISLLN VFTPQKTLEE FQDVYLVMEL MDANLCQVIH MELDHERMSY LLYQMLCGIKHLHSAGIIHR DLKPSNIVVK SDCTLKILDF GLARTACTNF MMTPYVVTRY YRAPEVILGM GYKENVDIWSVGCIMGELVK GCVIFQGTDH IDQWNKVIEQ LGTPSAEFMK KLQPTVRNYV ENRPKYPGIK FEELFPDWIFPSESERDKIK TSQARDLLSK MLVIDPDKRI SVDEALRHPY ITVWYDPAEA EAPPPQIYDA QLEEREHAIEEWKELIYKEV MDWEERSKNG VVKDQPSDAA VSSNATPSQS SSINDISSMS TEQTLASDTD SSLDASTGPLEGCRGGGSHH HHHH.
NCBI Acc Number NP_002743.3
Product Format 25mM HEPES at pH 8.0, 150mM NaCl, 2mM DTT and 10% glycerol. Physical appearance: Sterile Filtered clear solution.
Purity Greater than 85% as determined by SDS-PAGE. Mass spectrometry of tryptic digest products for identity determination.
Format 25mM HEPES at pH 8.0, 150mM NaCl, 2mM DTT and 10% glycerol.
Reconstitution and Storage Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. Avoid multiple freeze-thaw cycles.
Storage Store at 4 C if entire vial will be used within 2-4 weeks. Store, frozen at -20 C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for GWB-7B0204
Intended Use Research Use Only
Additional Information Unit Definition: Activity is approximately 200 units/mg. A certificate of analysis is provided, stating the enzymatic activity foreach production lot. 1.0 unit of activity is defined as the amount of enzyme required to catalyze the transfer ofumole of phosphate from ATP to to a protein substrate per minute at 30C.
:: Product Introduction: JUN N-terminal Kinase 2is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
Product Description: JNK2 is a soluble 48 kDa protein that is activated in response to cellular stress, radiation and growth factors. JNK2 binds to the amino terminal activation domains of c-jun or ATF2 regulating AP-1 transcriptional activity.This protein is the full-length form of the protein with a C-terminal His-tag (His-tag on COOH terminus).