JNK2a-2 Human (GWB-4CEB65)

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GWB-4CEB65

10-663-45557

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Description

Similarity: Contains 1 protein kinase domain.

Function: JNK2 isoforms display different binding patterns alpha-1 and alpha-2 preferentially bind to c-Jun whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it.

Additional Information

Name JNK2a-2 Human (GWB-4CEB65)
Related Product Names Mitogen-activated protein kinase 9, EC 2.7.11.24, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2, JNK-55, JNK2, JNK2A, JNK2B, PRKM9, JNK2BETA, p54aSAPK, JNK2ALPHA, MAPK9, JUN N-terminal Kinase 2 alpha 2, JNK2a-2.; EC 2.7.11.24; Stress-act
Description of Target Recombinant Human Jun N-terminal kinase 2, alpha 2
Gene Symbol JNK2a-2
Gene id 5601
Alias Symbols Mitogen-activated protein kinase 9, EC 2.7.11.24, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2, JNK-55, JNK2, JNK2A, JNK2B, PRKM9, JNK2BETA, p54aSAPK, JNK2ALPHA, MAPK9, JUN N-terminal Kinase 2 alpha 2, JNK2a-2, JNK2a-2 Hu
Swissprot ID P45984-1
Protein Name Mitogen-activated protein kinase 9
Protein Accession Num NP_001128516.1
Host E. Coli
NCBI Acc Number NP_002743.3
Product Format The protein concentration is 0.32mg/ml in 50mM Tris, pH 7.5, 0.15M NaCl, 0.27M sucrose, 10mM *-mercaptoethanol, 1mM EDTA and 0.1% Triton X-100. Physical appearance: Sterile Filtered clear solution.
Purity Greater than 95% as determined by SDS-PAGE.
Format The protein concentration is 0.32mg/ml in 50mM Tris, pH 7.5, 0.15M NaCl, 0.27M sucrose, 10mM ?-mercaptoethanol, 1mM EDTA and 0.1% Triton X-100.
Reconstitution and Storage Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. Avoid multiple freeze-thaw cycles.
Storage Store at 4 C if entire vial will be used within 2-4 weeks. Store, frozen at -20 C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for GWB-4CEB65
Intended Use Research Use Only
Additional Information Unit Definition: 200 units/mg. One unit of JNK22 activity is equal to 1nm of phosphate transferred to recombinant ATF-2 fusion protein per minute at 30C with a final ATP concentration of 100 uM. Recombinant active JNK2-2 is capable of autophosphorylation and also phosphorylates c-Jun at serine 63 and serine 73. Kinase activity may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application. JNK2-2 is longer than JNK2-1, and differs from JNK21 in that it contains additional C terminal sequences.
:: Product Introduction: JUN N-terminal Kinase 2is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
Product Description: JNK2 is a soluble 51 kDa protein (amino acids 2-424) that is activated in response to cellular stress, radiation and growth factors. JNK2 binds to the amino terminal activation domains of c-jun or ATF2 regulating AP-1 transcriptional activity.