POMC Over-expression Lysate Product (GWB-66BA00)

GWB-66BA00

36-614-470440

0.1 mg
$400.50
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Description

Expression cell line: Human HEK293T cell line

Epitope tag: C-terminal Myc/DDK* Detection Antibodies: Anti-DDK Antibody

Application: Western Blot experiments.
Left-Control; Right -Over-expression Lysate Description: Antigen standard for proopiomelanocortin (POMC), transcript variant 1 (transient overexpression lysate)

Components: 1 vial of 100 ug gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 ug empty vector transfected control cell lysate in RIPA buffer
1 vial of 0.5 ml 2xSDS Sample Buffer (4%SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 5% b-mercaptoethanol) Altered Expression of Regulators of the Cortical Chloride Transporters NKCC1 and KCC2 in Schizophrenia Arch Gen Psychiatry, Sep 2010; 10.1001/archgenpsychiatry.2010.114 [STK39]

Aquaporin-3 mediates hydrogen peroxide uptake to regulate downstream intracellular signaling PNAS, Sep 2010; 107: 15681 - 15686 [AQP8]

Coxsackievirus B3 Infection Activates the Unfolded Protein Response and Induces Apoptosis through Downregulation of p58IPK and Activation of CHOP and SREBP1 J. Virol., Sep 2010; 84: 8446 - 8459 [ATF6a]

Elevated cyclic AMP (cAMP) levels in T lymphocytes transformed by human T-cell lymphotropic virus type 1 (HTLV-1) J. Virol., Sep 2010; 84: 8732 - 8742 [PDE3B]

Additional Information

Name POMC Over-expression Lysate Product (GWB-66BA00)
Availability In Stock
Related Product Names POMC,ACTH; CLIP; LPH; MSH; NPP; POCPOMC Over-expression Lysate ProductPOMC
NCBI Acc Number NM_001035256
Molecular Weight 26.6 kDa
Application WB
Stability HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 15
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles.
Lysate samples can be diluted with 2xSDS Sample Buffer p
Datasheets/Manuals Printable datasheet for GWB-66BA00
Intended Use Research Use Only