Anti-mTOR Antibody

GWB-2FF98B

18-732-292496

100 μg
$490.00
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Description

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Mammalian target of rapamycin (mTOR) is a serine and threonine protein kinase that regulates numerous cellular functions, in particular, the initiation of protein translation. Rapamycin is a natural product macrolide that induces G1 growth arrest in yeast, Drosophila, and mammalian cells. mTOR has a long list of synonyms including FK506 binding protein12 - rapamycin associated protein 1, FK506 binding protein12 - rapamycin associated protein 2, FRAP1, FRAP2, RAFT1, RAPT1 and/or FKBP12-rapamycin associated protein (FRAP). mTOR is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, mTOR is a 245-kD protein referred to as RAFT1 with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of mTOR to FKBP12-rapamycin correlates with the ability of these ligands to inhibit cell cycle progression. Kristof,A.S. et al. (2003) Stimulation of signal transducer and activator of transcription-1 (STAT1)-dependent gene transcription by lipopolysaccharide and interferon-gamma is regulated by mammalian target of rapamycin. J. Biol. Chem. 278 (36), 33637-33644.

Additional Information

Name Anti-mTOR Antibody
Related Product Names Affinity Purified Anti-mTOR (Rabbit) Anti-mTOR
Gene Symbol MTOR
Gene Fullname Serine/threonine-protein kinase mTOR
Gene id 2475
Swissprot ID P42345
Protein Name Serine/threonine-protein kinase mTOR
Protein Accession Num NP_004949.1; XP_005263495.1
Nucleotide Accession Num NM_004958.3; XM_005263438.1
Clone Polyclonal
Host Rabbit
Immunogen The immunogen for anti-FRAP1 antibody: this affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 2440-2457 of human mTOR.
Homology Human; Mouse; Rat
Product Format Liquid (sterile filtered)
Applications ELISA, WB, IP
Application Info ELISA: 1:4,000 - 1:20,000 WB: 1:250 - 1:2,000
Format Liquid (sterile filtered)
Reconstitution and Storage Store vial at -20 C prior to opening.   This product is stable for several weeks at 4 C as an undiluted liquid.   Dilute only prior to immediate use.   For extended storage aliquot contents and freeze at -20 C or below.   Avoid cycles of freezing and thawing.   Expiration date is one (1) year from date of opening product.
Reactivity Human, Bovine, Mouse, Rat
Storage Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.
Datasheets/Manuals Printable datasheet for GWB-2FF98B
Intended Use Research Use Only
Key Reference Kristof,A.S. et al. (2003) Stimulation of signal transducer and activator of transcription-1 (STAT1)-dependent gene transcription by lipopolysaccharide and interferon-gamma is regulated by mammalian target of rapamycin. J. Biol. Chem. 278 (36), 33637-33644.Chen,Y., et al. (2003) Phospholipase D confers rapamycin resistance in human breast cancer cells. Oncogene 22 (25), 3937-3942.Nojima,H. et al. (2003) The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif. J. Biol. Chem. 278 (18), 15461-15464.
Additional Information Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
:: Label: Unconjugated
Preservative: 0.01% (w/v) Sodium Azide
Stabilizer: None