Anti-NFKB p50 (NFKB1) Antibody (GWB-CC6745)

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100 ul
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Antigen: p50 NFkB

Similarity: Contains 1 RHD (Rel-like) domain.

Fraction: Serum

Function: NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.

Additional Information

Name Anti-NFKB p50 (NFKB1) Antibody (GWB-CC6745)
Related Product Names anti-NFkB p50 (NFKB1) [Rabbit]; DNA-binding factor KBF1; EBP-1 Anti-NFKB p50 (NFKB1)NFKB1
Immunogen The immunogen for anti-NFKB1 antibody: human NFkB p50 (NFKB1) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Gene Symbol NFKB1
Gene Family ANKRD
Gene id 4790
Alias Symbols p50, KBF1, p105, EBP-1, NF-kB1, NFKB-p50, NFkappaB, NF-kappaB, NFKB-p105, NF-kappa-B,
Swissprot ID P19838
Protein Name Nuclear factor NF-kappa-B p105 subunit
Clone Polyclonal
Host Rabbit
Replacement This antibody may replace item sc-114 from Santa Cruz Biotechnology.
Homology Human
NCBI Acc Number NP_003989.2
Product Format Liquid (sterile filtered)
Application ELISA, WB, IP
Format Liquid (sterile filtered)
Reconstitution and Storage Store vial at -20 C or below prior to opening.   Dilute only prior to immediate use.   For extended storage, aliquot contents and freeze at -20 C or below.   Avoid cycles of freezing and thawing.   Expiration date is one (1) year from date of opening.
Reactivity Human, Mouse, Rat, Sheep
Application Info ELISA: 1:5,000 - 1:25,000 WB: 1:500 - 1:2,000
Storage -20C or Colder
Datasheets/Manuals Printable datasheet for GWB-CC6745
Species Reactivity Human
Intended Use Research Use Only
Additional Information Buffer: None
:: Label: Unconjugated
Preservative: 0.01% (w/v) Sodium Azide
Stabilizer: None