Rabbit anti p50 (NFkB1) subunit Antibody (GWB-675EEC)

GWB-675EEC

18-511-244478

0.1 ml
$655.00

Description

Similarity: Contains 1 RHD (Rel-like) domain.

Buffer: Not applicable

Function: NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.

Additional Information

Name Rabbit anti p50 (NFkB1) subunit Antibody (GWB-675EEC)
Availability Discontinued
Related Product Names Rabbit anti p50 (NFkB1) subunit; DNA-binding factor KBF1; EBP-1 Rabbit anti p50 (NFkB1) subunitNFKB1
Gene id 4790
Alias Symbols Rabbit Antibody to NFkB p50 subunit (NFkB1)
Swissprot ID P19838
Protein Name Nuclear factor NF-kappa-B p105 subunit
Protein Accession Num NP_003989.2
Host Rabbit
NCBI Acc Number NP_003989.2
Product Format Neat, Liquid
Specificity NFkB p50 (NFkB1) Subunit
May react non-specifically with other proteins. No reaction was observed against analogous mouse protein.
Purity Delipidation and defibrination. Product is sterile filtered.
Application WB
Application Data Immunogen: Human NFkB p50 (NFKB1) peptide corresponding to a region near the N-terminus of the human protein conjugated to KLH.
Clonality Polyclonal
Format Neat, Liquid
Reconstitution and Storage Store at -20C or below prior to opening. Prepare working dilution only prior to immediate use. For extended storage, aliquot and store at -20C or below. Avoid multiple freeze/thaw cycles. Expiration date is 6 months from date of opening pr
Storage Store at -20 C or below prior to opening. Prepare working dilution only prior to immediate use. For extended storage, aliquot and store at -20 C or below. Avoid multiple freeze/thaw cycles. Expiration date is 6 months from date of opening product.
Datasheets/Manuals Printable datasheet for GWB-675EEC
Intended Use Research Use Only
:: Buffer: Not applicable
Preservative: 0.01% (w/v) Sodium azide
Warnings: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes