PKC?, Active (GWB-2180A0)





Application Note: Kinase assay Recombinant full-length human PKCdelta was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Function: This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function.

Format: Recominatnt protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol

Catalytic Activity: ATP + a protein = ADP + a phosphoprotein.

Subunit: Interacts with PDK1 (By similarity). Interacts with RAD9A.

Subcellular Location: Cytoplasm (By similarity). Membrane; peripheral membrane protein (By similarity).

Ptm: Phosphorylated on Thr-507, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity (By similarity).

Similarity: Belongs to the Ser/Thr protein kinase family. PKC subfamily.

Similarity: Contains 1 C2 domain.

Similarity: Contains 2 phorbol-ester/DAG-type zinc fingers.

Similarity: Contains 1 protein kinase domain.

Summary: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [1] Zhao,Y., He,D., Saatian,B., Watkins,T., Spannhake,E.W., Pyne,N.J. and Natarajan,V.
Regulation of lysophosphatidic acid-induced epidermal growth factor receptor transactivation and interleukin-8 secretion in human bronchial epithelial cells by protein kinase Cdelta, Lyn kinase, and matrix metalloproteinases
[2] Iturrioz,X., Durgan,J., Calleja,V., Larijani,B., Okuda,H., Whelan,R. and Parker,P.J.
The von Hippel-Lindau tumour-suppressor protein interaction with protein kinase Cdelta
[3] Orosco,A., Fromigue,O., Hay,E., Marie,P.J. and Modrowski,D., et al.
Dual involvement of protein kinase C delta in apoptosis induced by syndecan-2 in osteoblasts
[4] Lee,C.F., Chen,Y.C., Liu,C.Y. and Wei,Y.H., et al.
Involvement of protein kinase C delta in the alteration of mitochondrial mass in human cells under oxidative stress
[5] Cronshaw,D.G., Kouroumalis,A., Parry,R., Webb,A., Brown,Z. and Ward,S.G.
Evidence that phospholipase-C-dependent, calcium-independent mechanisms are required for directional migration of T-lymphocytes in response to the CCR4 ligands CCL17 and CCL22
[6] Aris J.P., Basta P.V., Holmes W.D., Ballas L.M., Moomaw C., Rankl N.B., Blobel G., Loomis C.R., Burns D.J.
Molecular and biochemical characterization of a recombinant human PKC-delta family member.
[7] Hug H.
Partial cDNA Sequence of human protein kinase C delta.
[8] Nomoto S., Watanabe Y., Ninomiya-Tsuji J., Yang L.-X., Kikuchi K., Hagiwara M., Hidaka H., Matsumoto K., Irie K.
Functional analyses of mammalian protein kinase C isozymes in budding yeast and mammalian fibroblasts.
[9] Palmer R.H., Ridden J., Parker P.J.
Identification of multiple, novel, protein kinase C-related gene products.
[10] Yoshida K., Wang H.-G., Miki Y., Kufe D.
Protein kinase Cdelta is responsible for constitutive and DNA damage-induced phosphorylation of Rad9.

Additional Information

Name PKC?, Active (GWB-2180A0)
Related Product Names EC; nPKC-delta Protein kinase C delta typePRKCD
Swissprot ID Q05655
NCBI Acc Number NP_006245.2
Molecular Weight 0.5
Source Insect Cells
GI Number 31377782
Stability 1 year at -20 degree C.
Sequence Length 676
Storage Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
Datasheets/Manuals Printable datasheet for GWB-2180A0
Intended Use Research Use Only