PKC?, Active (GWB-165200)





Application Note: Kinase assay Recombinant full-length human PKCepsilon was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Function: This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.

Function: PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Catalytic Activity: ATP + a protein = ADP + a phosphoprotein.

Subunit: Forms a ternary complex with TRIM63 and GN2BL1 (By similarity).

Ptm: Phosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729.

Similarity: Belongs to the Ser/Thr protein kinase family. PKC subfamily.

Similarity: Contains 1 C2 domain.

Similarity: Contains 2 phorbol-ester/DAG-type zinc fingers.

Similarity: Contains 1 protein kinase domain.

Summary: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [1] Choi,D.S., Wang,D., Yu,G.Q., Zhu,G., Kharazia,V.N., Paredes,J.P., Chang,W.S., Deitchman,J.K., Mucke,L. and Messing,R.O.
PKCepsilon increases endothelin converting enzyme activity and reduces amyloid plaque pathology in transgenic mice
[2] LeHoux,J.G. and Lefebvre,A., et al.
Novel protein kinase C-epsilon inhibits human CYP11B2 gene expression through ERK1/2 signalling pathway and JunB
[3] Allen,T.R., Krueger,K.D., Hunter,W.J. III and Agrawal,D.K., et al.
Evidence that insulin-like growth factor-1 requires protein kinase C-epsilon, PI3-kinase and mitogen-activated protein kinase pathways to protect human vascular smooth muscle cells from apoptosis
[4] Lidington,E.A., Steinberg,R., Kinderlerer,A.R., Landis,R.C., Ohba,M., Samarel,A., Haskard,D.O. and Mason,J.C.
A role for proteinase-activated receptor 2 and PKC-epsilon in thrombin-mediated induction of decay-accelerating factor on human endothelial cells
[5] Kim,C.H., Braud,S., Isaac,J.T. and Roche,K.W., et al.
Protein kinase C phosphorylation of the metabotropic glutamate receptor mGluR5 on Serine 839 regulates Ca2+ oscillations
[6] Basta P., Strickland M.B., Holmes W., Loomis C.R., Ballas L.M., Burns D.J.
Sequence and expression of human protein kinase C-epsilon.
[7] Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., et al.
Generation and annotation of the DNA sequences of human chromosomes 2 and 4.
[8] Cenni V., Doeppler H., Sonnenburg E.D., Maraldi N., Newton A.C., Toker A.
Regulation of novel protein kinase C epsilon by phosphorylation.

Additional Information

Name PKC?, Active (GWB-165200)
Related Product Names EC; nPKC-epsilon PKCEProtein kinase C epsilon typePRKCE
Swissprot ID Q02156
NCBI Acc Number NP_005391.1
Molecular Weight 0.5
Source Insect Cells
GI Number 4885563
Stability 1 year at -20 degree C.
Sequence Length 737
Storage Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
Datasheets/Manuals Printable datasheet for GWB-165200
Intended Use Research Use Only