PKC?, Active (GWB-466B76)





Formulation: Recombinant protein stored in 50mM tris-HCl, pH 7.5 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25%glycerol.

Scientific Background : PKC-lamda is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases that can phosphorylate a wide variety of protein targets known to be involved in diverse cellular signaling pathways. In the brain, PKC-lamda is translocated to cell membranes during ischemia and is rapidly removed or degraded during the second otherwise lethal ischemic insult in preconditioned brains. This suggests that ischemic preconditioning enhances downregulation of cell signaling mediated by PKC-lamda and may thereby provide neuroprotection (1). Concentration: Lot specific and is stated on the vial Application Note: Kinase assay Recombinant full-length human PKCgamma was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Function: This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.

Function: PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Catalytic Activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain (By similarity).

Subunit: Interacts with CDCP1.

Disease: Defects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. SCA14 is an autosomal dominant neurodegenerative disase.

Similarity: Belongs to the Ser/Thr protein kinase family. PKC subfamily.

Similarity: Contains 1 C2 domain.

Similarity: Contains 2 phorbol-ester/DAG-type zinc fingers.

Similarity: Contains 1 protein kinase domain.

Summary: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). [1] Lim,J., Hao,T., Shaw,C., Patel,A.J., Szabo,G., Rual,J.F., Fisk,C.J., Li,N., Smolyar,A., Hill,D.E., Barabasi,A.L., Vidal,M., et al.
A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration
[2] Rosenberg,M. and Ravid,S., et al.
Protein kinase Cgamma regulates myosin IIB phosphorylation, cellular localization, and filament assembly
[3] Klebe,S., Durr,A., Rentschler,A., Hahn-Barma,V., Abele,M., Bouslam,N., Schols,L., Jedynak,P., Forlani,S., Denis,E.,, et al.
New mutations in protein kinase Cgamma associated with spinocerebellar ataxia type 14
[4] Seki,T., Adachi,N., Ono,Y., Mochizuki,H., Hiramoto,K., Amano,T., Matsubayashi,H., Matsumoto,M., Kawakami,H., Saito,N. and Sakai,N.
Mutant protein kinase Cgamma found in spinocerebellar ataxia type 14 is susceptible to aggregation and causes cell death
[5] Kim,C.H., Braud,S., Isaac,J.T. and Roche,K.W., et al.
Protein kinase C phosphorylation of the metabotropic glutamate receptor mGluR5 on Serine 839 regulates Ca2+ oscillations
[6] Cui W.C., Yu L., Chu Y.Y., Wang J., Zheng L.H., Zhou G.J., Zhao S.Y.
Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
[7] Coussens L., Parker P.J., Rhee L., Yang-Feng T.L., Chen E., Waterfield M.D., Francke U., Ullrich A.
Multiple, distinct forms of bovine and human protein kinase C suggest diversity in cellular signaling pathways.
[8] Kochs G., Hummel R., Meyer D., Hug H., Marme D., Sarre T.F.
Activation and substrate specificity of the human protein kinase C alpha and zeta isoenzymes.
[9] Al-Maghtheh M., Vithana E.N., Inglehearn C.F., Moore T., Bird A.C., Bhattacharya S.S.
Segregation of a PRKCG mutation in two RP11 families.
[10] Dryja T.P., McEvoy J., McGee T.L., Berson E.L.
No mutations in the coding region of the PRKCG gene in three families with retinitis pigmentosa linked to the RP11 locus on chromosome 19q.

Additional Information

Name PKC?, Active (GWB-466B76)
Related Product Names EC; PKC-gamma PKCGProtein kinase C gamma typePRKCG
Swissprot ID P05129
NCBI Acc Number NP_002730.1
Molecular Weight 0.5
Source Insect Cells
GI Number 13384594
Format 1ug in 10ul
Stability 1 year at -20 degree C.
Sequence Length 697
Storage Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable perforamce, avoid repeated handling and multiple freeze/thaw cycles.
Datasheets/Manuals Printable datasheet for GWB-466B76
Intended Use Research Use Only