CSK, Active (GWB-55A9CD)





Application Note: Kinase assay Description: Recombinant full-length human CSK was expressed by baculovirus in Sf9 insect using an N-terminal GST tag. Recombinant full-length human CSK was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Function: Specifically phosphorylates Tyr-504 on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.

Catalytic Activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit: Interacts with PTPN8 (By similarity). Interacts with phosphorylated SIT1, PAG1 and LIME1.

Subcellular Location: Cytoplasm (By similarity). Mainly cytoplasmic. Cell membrane (By similarity). Also present in lipid rafts (By similarity).

Tissue Specificity: Expressed in lung and macrophages.

Ptm: Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.

Similarity: Belongs to the Tyr protein kinase family. CSK subfamily.

Similarity: Contains 1 protein kinase domain.

Similarity: Contains 1 SH2 domain.

Similarity: Contains 1 SH3 domain. [1] Jiang,L.Q., Feng,X., Zhou,W., Knyazev,P.G., Ullrich,A. and Chen,Z., et al.
Csk-binding protein (Cbp) negatively regulates epidermal growth factor-induced cell transformation by controlling Src activation
[2] Johnsen,I.B., Nguyen,T.T., Ringdal,M., Tryggestad,A.M., Bakke,O., Lien,E., Espevik,T. and Anthonsen,M.W.
Toll-like receptor 3 associates with c-Src tyrosine kinase on endosomes to initiate antiviral signaling
[3] Lee,S., Ayrapetov,M.K., Kemble,D.J., Parang,K. and Sun,G., et al.
Docking-based substrate recognition by the catalytic domain of a protein tyrosine kinase, C-terminal Src kinase (Csk)
[4] Shah,K. and Vincent,F., et al.
Divergent roles of c-Src in controlling platelet-derived growth factor-dependent signaling in fibroblasts
[5] Wong,L., Lieser,S.A., Miyashita,O., Miller,M., Tasken,K., Onuchic,J.N., Adams,J.A., Woods,V.L. Jr. and Jennings,P.A.
Coupled motions in the SH2 and kinase domains of Csk control Src phosphorylation
[6] Partanen J., Armstrong E., Bergman M., Maekelae T.P., Hirvonen H., Huebner K., Alitalo K.
CYL encodes a putative cytoplasmic tyrosine kinase lacking the conserved tyrosine autophosphorylation site (Y416src).
[7] Braeuninger A., Holtrich U., Strebhardt K., Ruebsamen-Waigmann H.
Two additional protein-tyrosine kinases expressed in human lung: fourth member of the fibroblast growth factor receptor family and an intracellular protein-tyrosine kinase.
[8] Brauninger A., Holtrich U., Strebhardt K., Rubsamen-Waigmann H.
Isolation and characterization of a human gene that encodes a new subclass of protein tyrosine kinases.
[9] Braeuninger A., Karn T., Strebhardt K., Ruebsamen-Waigmann H.
Characterization of the human CSK locus.
[10] Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., et al.
Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201).

Additional Information

Name CSK, Active (GWB-55A9CD)
Related Product Names EC; C-SRC kinase; Protein-tyrosine kinase CYL Tyrosine-protein kinase CSKCSK
Swissprot ID P41240
NCBI Acc Number NP_004374.1
Molecular Weight 0.5
Source Insect Cells
GI Number 4758078
Format Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Background: CSK is a cytoplasmic tyrosine kinase that has been shown to downregulate the tyrosine kinase activity
Sequence Length 450
Storage Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
Datasheets/Manuals Printable datasheet for GWB-55A9CD
Intended Use Research Use Only