Description
Transient overexpression lysate of suppressor of cytokine signaling 3 (SOCS3)
Additional Information
| Name | SOCS3 Over-expression Lysate Product (GWB-C277F9) |
|---|---|
| Related Product Names | SOCS3,ATOD4; CIS3; Cish3; MGC71791; SOCS-3; SSI-3; SSI3SOCS3 Over-expression Lysate ProductSOCS3 |
| Gene Symbol | SOCS3 |
| Gene id | 9021 |
| Protein Accession Num | NP_003946 |
| Product Format | Liquid. 25mM Tris-HCl pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1x protein inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4 |
| Molecular Weight | 24.8 kDa |
| Application | WB |
| Stability | The lysate is stable for one year from date of shipping when stored at -80°C. |
| Reconstitution and Storage | Store the lysate at -80°C and avoid repeated freeze/ thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage. |
| Application Info | The antigen standards were validated by performing a Western blot using an anti-DDK antibody against the product in the right lane and the control lysate in the left lane. SDS-PAGE Sample preparation: Mix equal volume of overexpression lysates with 2X SDS sample buffer. Boil the sample for 10 minutes before loading. (In case of membrane proteins, incubate the mixture at room temperature for 30 minutes before loading.) |
| Datasheets/Manuals | Printable datasheet for GWB-C277F9 |
| Intended Use | Research Use Only |
| Additional Information | Components: 1 vial whole cell Lysate (SOCS3) (100 µg in 100 µl RIPA buffer) 1 vial vector transfected Lysate control (negative) (100 µg in 100 µl RIPA buffer) 1 vial SDS sample Buffer (250 µL) 2x |